ROLE OF PLATELET ACTIVATING FACTOR AND TUMOR NECROSIS FACTOR-ALPHA IN NEONATAL NECROTIZING ENTEROCOLITIS
ROLE OF PLATELET ACTIVATING FACTOR AND TUMOR NECROSIS FACTOR-ALPHA IN NEONATAL NECROTIZING ENTEROCOLITIS
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DOI：
10.1016/s0022-3476(05)80661-4
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发表时间：
1990-06-01
影响因子：
5.1
通讯作者：
HAGEMAN, JR
中科院分区：
文献类型：
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作者：
CAPLAN, MS;SUN, XM;HAGEMAN, JR
因为先前的研究表明血小板活化因子和肿瘤坏死因子-α（TNF-α）是大鼠实验性坏死性小肠结肠炎的重要介质，我们测量了血小板活化因子、乙酰水解酶（血小板活化因子分解酶）和TNF-α。在12名患有坏死性小肠结肠炎的新生儿和8名年龄匹配的对照受试者的血浆中，这些受试者具有相似的胎龄、出生后年龄和体重。几乎所有坏死性小肠结肠炎患者的血浆血小板活化因子值均升高（18.1 ± 0.01）。3.6 ng/ml vs. 3.1 . ±. 0.9 ng/ml/min，在对照受试者中，p < 0.01）。患者的血浆乙酰水解酶活性低于对照组（10.6 ± 0.01）。0.7 nmol/ml/min vs. 23.0 ±. 1.4 nmol/ml/min，p < 0.01）。血浆TNF-α浓度在坏死性小肠结肠炎患者中显著升高（136 . ±. 75 U/ml vs 1.5 . ±. 0.8 U/ml，p < 0.05），但个体差异较大。在个体TNF-α之间没有相关性。和血小板活化因子水平。我们得出结论，血小板活化因子和TNF-α。在患有坏死性小肠结肠炎的患者中升高，并且抑制的血小板活化因子降解有助于血小板活化因子水平的升高;血小板活化因子和TNF-α。可能与坏死性小肠结肠炎的病理生理有关。
Because previous investigations have suggested that platelet activating factor and tumor necrosis factor-alpha (TNF-.alpha.) are important mediators of experimental necrotizing enterocolitis in the rat, we measured platelet activating factor, acetylhydrolase (the platelet activating factor breakdown enzyme) and TNF-.alpha. in the plasma of 12 human neonates with necrotizing enterocolitis and eight age-matched control subjects with similar gestational ages, postnatal ages, and weights. Almost all patients with necrotizing enterocolitis had elevated plasma platelet activating factor values (18.1 .+-. 3.6 ng/ml vs. 3.1 .+-. 0.9 ng/ml/min, in control subjects, p < 0.01). Plasma acetylhydrolase activity was lower in patients than in control subjects (10.6 .+-. 0.7 nmol/ml/min vs. 23.0 .+-. 1.4 nmol/ml/min, p < 0.01). Plasma TNF-.alpha. concentration was significantly elevated in patients with necrotizing enterocolitis (136 .+-. 75 U/ml vs 1.5 .+-. 0.8 U/ml, p < 0.05), although the individual variation was high. There was no correlation between individual TNF-.alpha. and platelet activating factor levels. We conclude that platelet activating factor and TNF-.alpha. are elevated in patients with necrotizing enterocolitis and that suppressed platelet activating factor degradation contributes to the increased platelet activating factor levels; platelet activating factor and TNF-.alpha. may contribute to the pathophysiology of necrotizing enterocolitis.