MOLECULAR-CLONING OF LYMPHADENOPATHY-ASSOCIATED VIRUS
MOLECULAR-CLONING OF LYMPHADENOPATHY-ASSOCIATED VIRUS
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DOI：
10.1038/312757a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
WAINHOBSON, S
中科院分区：
文献类型：
--
作者：
ALIZON, M;SONIGO, P;WAINHOBSON, S
淋巴结病相关病毒（LAV）是一种人类逆转录病毒，首先从患有淋巴结病综合征的同性恋患者中分离出来，通常是获得性免疫缺陷综合征（AIDS）的前驱症状或良性形式。后来从艾滋病患者或艾滋病前期患者中分离出了其他LAV分离株3 - 5，所有可用数据均表明该病毒是艾滋病的病原体。该病毒在活化的T淋巴细胞上增殖，并对T细胞亚群OKT 4具有嗜性（参考文献6），在该亚群中诱导细胞病变效应。LAV的主要核心蛋白与其他已知的逆转录病毒抗原在抗原性上无关1，2，7。LAV样病毒最近已独立地从艾滋病患者和艾滋病前期患者中分离出来。这些病毒被称为人类T细胞白血病/淋巴瘤病毒III型（HTLV-III）8- 11和艾滋病相关逆转录病毒（ARV）12，似乎与LAV有许多共同的特征，可能代表LAV原型的独立分离株。我们试图通过分子克隆其基因组来表征LAV。用克隆的LAV互补DNA筛选由LAV感染的T淋巴细胞基因组DNA构建的重组cDNA文库。表征了两个家族的克隆，其在限制性位点上不同。病毒基因组比任何其他人类逆转录病毒基因组都长（9.1-9.2个酶）。
Lymphadenopathy-associated virus (LAV) is a human retrovirus first isolated1from a homosexual patient with lymphadenopathy syndrome, frequently a prodrome or a benign form of acquired immune deficiency syndrome (AIDS)2. Other LAV isolates have subsequently been recovered from patients with AIDS or pre-AIDS3–5and all available data are consistent with the virus being the causative agent of AIDS. The virus is propagated on activated T lymphocytes and has a tropism for the T-cell subset OKT4 (ref. 6), in which it induces a cytopathic effect. The major core protein of LAV is antigenically unrelated to other known retroviral antigens1,2,7. LAV-like viruses have more recently been independently isolated from patients with AIDS and pre-AIDS. These viruses, called human T-cell leukaemia/lymphoma virus type III (HTLV-III)8–11and AIDS-associated retrovirus (ARV)12, seem to have many characteristics in common with LAV and probably represent independent isolates of the LAV prototype. We have sought to characterize LAV by the molecular cloning of its genome. A cloned LAV complementary DNA was used to screen a library of recombinant phages constructed from the genomic DNA of LAV-infected T lymphocytes. Two families of clones were characterized which differ in a restriction site. The viral genome is longer than any other human retroviral genome (9.1–9.2 kilobases).