Protective effect of gallic acid isolated from Peltiphyllum peltatum against sodium fluoride-induced oxidative stress in rat's kidney
Protective effect of gallic acid isolated from Peltiphyllum peltatum against sodium fluoride-induced oxidative stress in rat's kidney
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DOI：
10.1007/s11010-012-1464-y
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发表时间：
2013-01-01
影响因子：
4.3
通讯作者：
Amani, Mohammad Ali
中科院分区：
文献类型：
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作者：
Nabavi, Seyed Mohammad;Habtemariam, Solomon;Amani, Mohammad Ali
在本研究中，没食子酸分离盾叶甲在氟化钠（NaF）处理的大鼠的肾保护作用进行了研究。通过饮用水以600 ppm的NaF中毒1周诱导肾毒性。测定肾组织匀浆中硫代巴比妥酸反应物、还原型谷胱甘肽含量以及超氧化物歧化酶和过氧化氢酶活性。检测肾损伤的血清生化指标，包括肌酐、血清尿素、血尿素氮、尿酸水平以及磷酸盐和钙水平。NaF中毒导致肾组织匀浆中硫代巴比妥酸反应物质水平显著增加（与对照组相比为46%），谷胱甘肽浓度降低（47%），超氧化物歧化酶（46%）和过氧化氢酶（41%）活性降低。氟化钠中毒还引起肾脏生化标志物的显著改变，增加尿素、尿酸、血尿素氮、肌酐和磷酸盐的水平，并降低钙的水平。在NaF中毒前1周每天给予没食子酸（20 mg/kg），抗氧化剂-氧化剂平衡与NaF未处理组相似。水飞蓟素，使用标准的抗氧化剂，也表现出肾保护活性。我们的结论是，氟化钠引起肾毒性和氧化应激在肾组织和每天给药没食子酸中毒前1周抑制毒性和氧化应激。
In the present study, the nephroprotective effect of gallic acid isolated from Peltiphyllum peltatum was examined in sodium fluoride (NaF) treated rats. Nephrotoxicity was induced by 1-week intoxication of NaF at 600 ppm through drinking water. The levels of thiobarbituric acid reactive substances, reduced glutathione as well as activities of superoxide dismutase and catalase in renal tissues homogenates were determined. The serum biochemical markers of renal injuries including creatinine, serum urea, blood urea nitrogen, uric acid levels as well as the levels of phosphate and calcium were also assessed. Intoxication with NaF caused a significant increase in the levels of thiobarbituric acid reactive substances (46 % versus to control) and reduced the glutathione concentration (47 %) and the activities of superoxide dismutase (46 %) and catalase (41 %) in renal tissues homogenates. NaF intoxication also induced significant alterations in the kidney biochemical markers increasing the levels of urea, uric acid, blood urea nitrogen, creatinine, and phosphate and decreasing the levels of calcium. Daily administration of gallic acid (20 mg/kg) for 1 week before NaF intoxication brought the antioxidant-oxidant balance similar to the NaF-untreated group. Silymarin, used a standard antioxidant agent, also showed a nephroprotective activity. We concluded that NaF caused nephrotoxicity and oxidative stress in renal tissues and daily administration of gallic acid for 1 week prior to intoxication inhibited toxicity and oxidative stress.