Spectral karyotyping of fresh, non-inseminated oocytes.
Spectral karyotyping of fresh, non-inseminated oocytes.
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新鲜、未受精卵母细胞的光谱核型分析。
DOI：
10.1093/molehr/8.6.580
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发表时间：
2002
影响因子：
4
通讯作者：
Munné,Santiago
中科院分区：
文献类型：
--
作者：
Sandalinas,Mireia;Márquez,Carmen;Munné,Santiago
本研究的目的是通过分析未受精和未受精的新鲜人类卵母细胞的染色体，来确定女性减数分裂Ⅰ中非整倍体的产生机制。为此目的，从16个卵母细胞供体（24-48岁）获得131个新鲜卵母细胞。这些卵母细胞在回收后立即固定，并通过光谱核型分析对来自13个供体的47个优质中期分裂相进行分析，以识别所有23种染色体类型。数据分为两个母亲年龄组，24-34岁（n= 31）和16 - 35岁（n= 16）。年龄越大，不分离（13和25%）、不平衡预分裂（10和44%，P < 0.01）和平衡预分裂（6和62%，P < 0.001）的发生率越高。随着染色体大小的减小，平衡预分裂率增加（P< 0.001）。目前的结果是第一次在新鲜卵母细胞中获得的，其中所有的染色体都被特别确定，并支持以前的理论，即染色单体的预分裂是导致非整倍体的一个主要因素。以前的报告与受精，未受精的卵母细胞固定24小时后检索遭受人工预分裂的染色单体引发的卵母细胞在体外老化。
The object of this study was to determine the mechanisms producing aneuploidy in female meiosis I by analysing the whole chromosome complement of human non-inseminated and unfertilized fresh oocytes. For this purpose, 131 fresh oocytes were obtained from 16 oocyte donors (24–48 years old). These oocytes were fixed immediately after retrieval and 47 good quality metaphases from 13 donors were analysed by spectral karyotyping to identify all 23 chromosome types. The data was divided into two maternal age groups, 24–34 (n= 31) and │35 years (n= 16). More non-disjunction (13 and 25%), unbalanced predivision (10 and 44%,P< 0.01) and balanced predivision (6 and 62%,P< 0.001) events were found in the older group of oocytes. There was an increase in balanced predivision with decreasing chromosome size (P< 0.001). The present results are the first obtained in fresh oocytes where all chromosomes were specifically identified, and support previous theories that predivision of chromatids is a major factor causing aneuploidy. Previous reports with inseminated, non-fertilized oocytes fixed │24 h after retrieval suffered from artefactual predivision of chromatids triggered by in-vitro ageing of oocytes.