Specific lysis of human colon tumor cells by antibodies to CEA and isoantigen A: dependence on rabbit serum or neuraminidase.
Specific lysis of human colon tumor cells by antibodies to CEA and isoantigen A: dependence on rabbit serum or neuraminidase.
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CEA 和同种抗原 A 抗体对人结肠肿瘤细胞的特异性裂解：依赖于兔血清或神经氨酸酶。
DOI：
10.4049/jimmunol.117.5_part_2.1943
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发表时间：
1976
影响因子：
4.4
通讯作者：
W. Rawls
中科院分区：
文献类型：
--
作者：
W. Tompkins;P. Seth;Dick;J. L. Palmer;S. Gee;W. Rawls
应用~（51）Cr细胞毒性试验检测人结肠癌细胞表面癌胚抗原（CEA）和同种抗原A的特异性抗体反应。当使用人血清或豚鼠血清作为补体来源时，尽管特异性抗体结合并至少通过细胞表面上的C3组分激活补体，但未证实抗CEA或抗同种抗原A的细胞毒性。相反，当兔血清用作补体来源时，证明了特异性抗CEA和抗同种抗原A的细胞毒性。如果在试验前用神经氨酸酶处理细胞，则豚鼠补体也可实现特异性抗体介导的细胞溶解。这些结果支持这样的概念，即某些肿瘤细胞具有使它们对免疫裂解具有抗性的表面性质。
The 51Cr cytotoxicity test was used to measure specific antibody reactions against carcinoembryonic antigen (CEA) and isoantigen A on the surface of human colon tumor cells. When human serum or guinea pig serum was used as a source of complement, no anti-CEA or anti-isoantigen A cytotoxicity was demonstrable despite binding of specific antibodies and activation of complement at least through the C3 component on the cell surface. In contrast, specific anti-CEA and anti-isoantigen A cytotoxicity was demonstrated when rabbit serum was used as a source of complement. Specific antibody-mediated cell lysis was also achieved with guinea pig complement if the cells were treated with neuraminidase before testing. These results support the concept that certain tumor cells have surface properties that render them resistant to immune lysis.