Intergenerational reproductive toxicity of chlordecone in male Caenorhabditis elegans
Intergenerational reproductive toxicity of chlordecone in male Caenorhabditis elegans
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DOI：
10.1007/s11356-019-04519-1
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发表时间：
2019-02
影响因子：
5.8
通讯作者：
Shuhao Dai;Ying Zhang;Y. Miao;Ran Liu;Yuepu Pu;L. Yin
中科院分区：
文献类型：
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作者：
Shuhao Dai;Ying Zhang;Y. Miao;Ran Liu;Yuepu Pu;L. Yin
十氯酮（CLD），又称开蓬，是一种合成有机氯农药。CLD是自然界中常见的持久性有机污染物之一，对环境和人类健康有着深远的影响。本研究旨在探讨CLD对雄性秀丽隐杆线虫及其子代的生殖毒性效应。将L1期雄性线虫暴露于对照组（M9溶液）和4个剂量组（0.02、0.2、2和20 μg/L）。暴露48 h后，挑取雄性线虫进行交配实验和子代实验，统计子代数、父本观察时间和F1代观察时间;用二巯基苯肼（DAPI）染色计数雄性线虫减数分裂中的生殖细胞数和精子数，并在显微镜明视野下观察线虫腺区。雄性线虫的子代数分别为351.20 ± 31.40、321.60 ± 24.70、307.30 ± 19.30、240.10 ± 27.60和227.90 ± 22.70（P< 0.05），世代时间分别为55.80 ± 1.95 h、56.40 ± 1.60 h、56.70 ± 0.92 h、60.80 ± 0.95 h、69.60 ± 1.97 h（P< 0.05），性腺相对面积分别为（99.80 ± 6.27）%，（93.00 ± 1.70）%，（85.00 ± 1.70）%，（70.70 ± 9.81）%，和（60.00 ± 5.23）%（P< 0.05）; DAPI染色结果显示，减数分裂区生殖细胞数分别为191.00 ± 10.97、181.10 ± 15.56、177.00 ± 9.20、147.50 ± 10.56，精子数分别为335.60 ± 21.31、308.60 ± 19.60、306.00 ± 11.23、260.10 ± 27.41和255.00 ± 3.72（P< 0.05）。F_1代的后代数分别为328.10 ± 22.28、167.50 ± 15.30、150.00 ± 13.65、131.30 ± 18.40和130.20 ± 16.17（P< 0.05），世代时间分别为55.50 ± 2.36、71.10 ± 0.97、70.90 ± 0.52、74.10 ± 2.07和73.90 ± 1.35 h（P< 0.05）。各组按M9溶液、0.02、0.2、2和20 μg/L的顺序分组。结果表明，CLD可导致雄性线虫的后代数、生殖腺相对面积、生殖细胞数和精子数减少，世代时间延长。在没有CLD的后代中，CLD对世代时间和精子数量的影响仍然可以在后代中观察到。总之，CLD诱导雄性线虫生殖毒性，并导致后代缺陷。
Chlordecone (CLD), also named Kepone, is a synthetic organochlorine pesticide. As one of the common persistent organic pollutants (POPs) in nature, CLD has a profound impact on the environment and human health. The study aims to investigate the reproductive toxicity effects of CLD on maleCaenorhabditis elegansand on progeny. L1-stage male nematodes were exposed to the control group (M9 solution) and four dose groups (0.02, 0.2, 2, and 20 μg/L). After exposure for 48 h, the male nematodes were picked to mating experiment and progeny experiment that the number of progeny and the time of observation in male parent and in F1 generation were counted; the number of germ cells and the number of sperm in the meiotic division of male nematodes were counted by staining with dimercaptophenyl hydrazine (DAPI), and the nematode gland area was observed under the bright field of the microscope. In male nematodes, the results showed that a number of progeny were 351.20 ± 31.40, 321.60 ± 24.70, 307.30 ± 19.30, 240.10 ± 27.60, and 227.90 ± 22.70 (P< 0.05); the generation times were 55.80 ± 1.95 h, 56.40 ± 1.60 h, 56.70 ± 0.92 h, 60.80 ± 0.95 h, and 69.60 ± 1.97 h (P< 0.05); relative areas of gonad were (99.80 ± 6.27)%, (93.00 ± 1.70)%, (85.00 ± 1.70)%, (70.70 ± 9.81)%, and (60.00 ± 5.23)% (P< 0.05); DAPI staining results showed the number of germ cells in meiosis area were 191.00 ± 10.97, 181.10 ± 15.56, 177.00 ± 9.20, 147.50 ± 10.56, and 139.30 ± 23.79 (P< 0.05); the sperm numbers were 335.60 ± 21.31, 308.60 ± 19.60, 306.00 ± 11.23, 260.10 ± 27.41, and 255.00 ± 3.72 (P< 0.05). In the F1 generation, the progeny numbers were 328.10 ± 22.28, 167.50 ± 15.30, 150.00 ± 13.65, 131.30 ± 18.40, and 130.20 ± 16.17 (P< 0.05); the generation times were 55.50 ± 2.36, 71.10 ± 0.97, 70.90 ± 0.52, 74.10 ± 2.07, and 73.90 ± 1.35 h (P< 0.05). The groups are grouped in order as M9 solution, 0.02, 0.2, 2, and 20 μg/L. The results revealed that CLD caused decrease in progeny number, relative area of gonad, number of germ cells, and sperm number and prolonged the generation time in the male nematode. In offspring grown up without CLD, the effect of CLD on generation time and sperm number can still be observed on offspring. In conclusion, CLD induces male nematode reproductive toxicity and causes defects in offspring.