EXPRESSION OF CSF-1/C-FMS AND SF/C-KIT MESSENGER-RNA DURING PREIMPLANTATION MOUSE DEVELOPMENT
EXPRESSION OF CSF-1/C-FMS AND SF/C-KIT MESSENGER-RNA DURING PREIMPLANTATION MOUSE DEVELOPMENT
复制标题
DOI：
10.1016/0012-1606(92)90207-w
复制
发表时间：
1992-05-01
影响因子：
2.7
通讯作者：
POLLARD, JW
中科院分区：
文献类型：
--
作者：
ARCECI, RJ;PAMPFER, S;POLLARD, JW
c-fms和c-kit分别是集落刺激因子-1（CSF-1）和steel因子（SF）的结构相关的酪氨酸激酶受体。与c-kitmRNA一样，c-fmsmRNA的水平在卵母细胞成熟期间增加，并且是在未受精卵母细胞中发现的丰富的母体信息。受精后，c-fms和c-kitoocyte mRNA的水平迅速下降，直到它们在早期2细胞阶段不再被检测到。到2细胞晚期，两种mRNA都重新表达，尽管水平较低。这种低水平的mRNA表达在整个着床前发育过程中持续存在。CSF-1和SF的成绩单中未检测到早期植入前胚胎，但在卵丘细胞，输卵管，和子宫中检测到，这表明这些生长因子的旁分泌作用在植入前期间。CSF-1/c-fmsmRNA和SF/c-kitmRNA的表达模式与这两种配体/受体系统可能在植入前发育期间以补偿或协同方式起作用的假设一致。
c-fmsand c-kitare structurally related tyrosine kinase receptors for colony-stimulating factor-1 (CSF-1) and forsteelfactor (SF), respectively. The level of c-fmsmRNA, like c-kitmRNA, increases during the maturation of oocytes and is an abundant maternal message found in the unfertilized oocyte. Following fertilization, the level of both c-fmsand c-kitoocyte mRNAs decreases rapidly until they are no longer detected by the early 2-cell stage. By the late 2-cell stage, both mRNAs are reexpressed, albeit at low levels. This low level of mRNA expression continues throughout preimplantation development. CSF-1 and SF transcripts are not detected in early preimplantation embryos, but are detected in cumulus cells, oviduct, and uterus, suggesting a paracrine action of these growth factors during the preimplantation period. The patterns of CSF-1/c-fmsmRNA and SF/c-kitmRNA expression are consistent with the hypothesis that these two ligand/receptor systems may act in a compensatory or synergistic manner during preimplantation development.