ISOPRENOID BIOSYNTHESIS IN BACTERIA - 2 DIFFERENT PATHWAYS
ISOPRENOID BIOSYNTHESIS IN BACTERIA - 2 DIFFERENT PATHWAYS
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DOI：
10.1111/j.1574-6968.1993.tb06375.x
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发表时间：
1993-08-01
影响因子：
2.1
通讯作者：
WELLE, R
中科院分区：
文献类型：
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作者：
HORBACH, S;SAHM, H;WELLE, R
异戊烯焦磷酸是类异戊二烯形成的重要中间产物，本文研究了六种不同细菌的生物合成。黄色粘球菌、肉色葡萄球菌、植物乳杆菌和表皮嗜盐杆菌的无细胞提取物将[C-14]乙酰辅酶A或[C-14]羟甲基戊二酰辅酶A转化为[C-14]甲羟戊酸。此外，[C-14]甲羟戊酸、[C-14]甲羟戊酸-5-磷酸和[C-14]甲羟戊酸-5-焦磷酸代谢为[C-14]异戊烯焦磷酸。这些数据证明了在细菌中形成异戊烯焦磷酸的乙酰乙酸途径的体外操作。与此相反，使用运动发酵单胞菌和大肠杆菌的无细胞提取物，没有检测到该反应序列的中间体。这些结果表明，在细菌中存在至少两种不同的异戊烯焦磷酸生物合成途径。
The biosynthesis of isopentenylpyrophosphate, a central intermediate Of isoprenoid formation, was investigated in six different bacterial organisms. Cell-free extracts of Myxococcus fulvus, Staphylococcus carnosus, Lactobacillus plantarum and Halobacterium cutirubrum converted [C-14]acetyl-CoA or [C-14]hydroxymethylglutaryl-CoA to [C-14]Mevalonic acid. Furthermore, [C-14]mevalonic acid, [C-14]mevalonate-5-phosphate and [C-14]mevalonate-5-pyrophosphate were metabolized to [C-14]isopentenylpyrophosphate. These data demonstrated the in vitro operation of acetoacetate pathway for the formation of isopentenylpyrophosphate in bacteria. In contrast, no intermediates of this reaction sequence could be detected using cell-free extracts of Zymomonas mobilis and Escherichia coli. These results indicate that at least two different pathways for the biosynthesis of isopentenylpyrophosphate are present in bacteria.