The effects of isoliquiritigenin on endometriosis in vivo and in vitro study
The effects of isoliquiritigenin on endometriosis in vivo and in vitro study
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DOI：
10.1016/j.phymed.2020.153214
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发表时间：
2020-10-01
期刊：
影响因子：
7.9
通讯作者：
Hsia, Shih-Min
中科院分区：
文献类型：
--
作者：
Hsu, Yi-Wen;Chen, Hsin-Yuan;Hsia, Shih-Min
背景：子宫内膜异位症是一种常见的妇科疾病，其特征是子宫内膜组织在子宫腔外、卵巢、输卵管和盆腔腹膜上生长。异甘草素（ISL）是从甘草（glycyrhiza uralensis）和葱（Allium cepa）的根中分离得到的一种天然类黄酮。ISL先前已显示出抗氧化、抗炎、抗增殖和抗肿瘤活性。目的：探讨ISL在体内和体外对子宫内膜异位症的影响。方法：用ISL和β -雌二醇处理End1/E6E7子宫内膜异位症细胞。MTT法检测细胞活力。通过伤口愈合实验评估细胞迁移。western blot检测上皮-间充质转化相关蛋白的表达。雌性Balb/c小鼠通过子宫组织移植腹腔手术诱导子宫内膜异位症，用ISL或载药治疗4周。随后通过高分辨率超声成像分析病变生长情况。ELISA法检测血清及病灶炎性因子。western blot检测子宫内膜异位症病变组织中emt相关蛋白和凋亡相关蛋白的表达。结果：ISL处理抑制了End1/E6E7的生存能力和迁移能力。ISL处理增加了E-cadherin的表达，降低了N-cadherin、Slug和Snail的表达。在动物模型中，ISL治疗减少了子宫内膜异位症病变的体积和重量，降低了血清和病变炎性细胞因子，抑制了EMT，诱导了病变的凋亡。结论：ISL可抑制End1/E6E7细胞的活力、迁移和EMT相关蛋白，减少子宫内膜异位症病变的体积和重量，抑制炎症因子和EMT，诱导病变细胞凋亡，改善子宫内膜异位症。
Background: Endometriosis is a common gynaecological disease characterized by growth of uterine endometrial tissue, outside the uterine cavity, on the ovaries, oviduct and pelvic peritoneum. Isoliquiritigenin (ISL) is a natural flavonoid isolated from the root of licorice (Glycyrrhiza uralensis) and shallot (Allium cepa). ISL has previously shown antioxidant, anti-inflammatory, anti-proliferation and anti-tumor activities.Purpose: This study aimed to investigate the effects of ISL on endometriosis in vivo and in vitro.Methods: End1/E6E7 endometriosis cells were treated with ISL and beta-estradiol. The MTT assay was used to detect cell viability. Cell migration was evaluated by the wound-healing assay. The expression of epithelial-tomesenchymal transition (EMT)-related proteins were detected by western blot. Female Balb/c mice, surgically induced to have endometriosis by transplanting uterine tissue into the abdominal cavity, were treated with ISL or vehicle for 4 weeks. Lesion growth was subsequently analyzed by high-resolution ultrasound imaging. Serum and lesion inflammatory cytokines were measured by ELISA. EMT-related proteins and apoptosis-related proteins of endometriotic lesions were detected by western blot.Results: It was observed that ISL treatment inhibited the viability and migration of End1/E6E7. ISL treatment increased the expression of E-cadherin, and decreased the expression of N-cadherin, Slug and Snail. In the animal model, ISL treatment reduced the volume and weight of endometriotic lesions, decreased serum and lesion inflammatory cytokines, inhibited EMT, and induced apoptosis of the lesions.Conclusion: ISL inhibited the viability, migration and EMT-related proteins of End1/E6E7 cells, reduced the volume and weight of endometriotic lesions, inhibited inflammatory cytokines and EMT, and induced apoptosis of the lesions to improve endometriosis.