Elevated Levels of Authentic Plasma Hydroperoxides in NIDDM
Elevated Levels of Authentic Plasma Hydroperoxides in NIDDM
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DOI：
10.2337/diab.44.9.1054
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发表时间：
1995-09
期刊：
影响因子：
7.7
通讯作者：
J. Nourooz-Zadeh;J. Tajaddini-Sarmadi;S. McCarthy;D. Betteridge;S. Wolff
中科院分区：
文献类型：
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作者：
J. Nourooz-Zadeh;J. Tajaddini-Sarmadi;S. McCarthy;D. Betteridge;S. Wolff
使用一种精确的技术来测量真实的血浆脂质过氧化氢(ROOHs)，我们发现非胰岛素依赖型糖尿病(NIDDM)患者的RoOH水平高于对照组。用二甲酚橙氧化亚铁与选择性RoOH还原剂三苯基膦联用测定ROOHs。在560 nm波长处测定了二甲酚橙铁络合物的形成，并与过氧化氢进行了校正。22例非胰岛素依赖型糖尿病患者血药浓度为9.04±4.3Mol/L(Mean±SD)μ。这一浓度较高(P0.1)。NIDDM组维生素E水平(9.03±3.31vs.10.31±5.02μg/ml)呈下降趋势，但未能达到95%可信水平。糖尿病组的血浆ROOHs与总胆固醇、甘油三酯、空腹血糖、HbA1、维生素E或TBARM水平无关。这些数据表明，测量真实的ROOHs表明NIDDM与氧化应激有关，这可能与脂代谢和血糖控制的异常无关。
Using a precise technique for measuring authentic plasma lipid hydroperoxides (ROOHs), we show that individuals with non-insulin-dependent diabetes mellitus (NIDDM) have higher levels of ROOH than do control subjects. ROOHs were measured by the ferrous oxidation with xylenol orange assay coupled with the selective ROOH reductant triphenylphosphine. Formation of the ferric xylenol orange complex was determined at 560 nm and calibrated against H2O2. For 22 individuals with NIDDM, a concentration of 9.04 ± 4.3 μmol/l (mean ± SD) ROOH was recorded. This concentration was higher (P 0.1). A trend to lower vitamin E levels in the NIDDM group (9.03 ± 3.31 vs. 10.31 ± 5.02 μg/ml in control subjects) failed to achieve significance at the 95% confidence level. Plasma ROOHs in the diabetic group did not correlate with total plasma cholesterol, triglyceride, fasting glucose, HbA1, vitamin E, or TBARM levels. These data indicate that measurement of authentic ROOHs shows NIDDM to be associated with oxidative stress, which may be unrelated to abnormalities in lipid metabolism and glycemic control.