Differentiation of dextran-producing Leuconostoc strains from fermented rice cake (puto) using pulsed-field gel electrophoresis.
Differentiation of dextran-producing Leuconostoc strains from fermented rice cake (puto) using pulsed-field gel electrophoresis.
复制标题
使用脉冲场凝胶电泳从发酵米饼 (puto) 中分化产生葡聚糖的明串珠菌菌株。
DOI：
10.1016/0168-1605(94)00146-w
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发表时间：
1995
影响因子：
5.4
通讯作者：
C.
中科院分区：
文献类型：
--
作者：
W. Kelly;R. V. Asmundson;G. L. Harrison;C.
乳酸菌是从菲律宾的一种发酵米糕中分离出来的，这种发酵米糕在菲律宾被用作早餐和零食。微生物区系以产生葡聚糖的明胶螺菌为主，利用限制性内切酶酶切染色体DNA的脉冲场凝胶电泳法结合分类试验将其分为四个组。这四个群分别对应于肠系膜明串珠菌亚种。肠系膜杆菌、拟肠状明串珠菌、柠檬酸明串珠菌和黄色明串珠藻。有几个菌株表现出不寻常的聚集表型，其中两个菌株能够抑制其他菌株的乳酸菌。
Lactic acid bacteria were isolated from puto, a fermented rice cake consumed as a breakfast and snack food in the Philippines. The microflora was dominated by dextran-producing leuconostocs, and these were differentiated into four groups using pulsed-field gel electrophoresis of restriction enzyme digested chromosomal DNA, in conjunction with taxonomic tests. The four groups corresponded to the species Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc pseudomesenteroides, Leuconostoc citreum and Leuconostoc fallax. Several strains showed an unusual clumping phenotype, and two of these were capable of inhibiting other strains of lactic acid bacteria.