Cyclic AMP mediates the cell cycle dynamics of energy metabolism in Saccharomyces cerevisiae
Cyclic AMP mediates the cell cycle dynamics of energy metabolism in Saccharomyces cerevisiae
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作者：
Müller, D;Exler, S;Reuss, M
我们研究了3 '，5'-环腺苷酸（cAMP）在酿酒酵母同步培养和振荡连续培养中介导能量代谢和细胞周期进程之间耦合的作用。第一次，细胞内cAMP的峰值显示之前观察到的细胞周期相关的振荡过程中的海藻糖和糖原的分解。在同步培养物中的测量表明，这个峰值可以与cAMP的细胞周期动力学条件下的葡萄糖限制的生长，这被发现显着不同，从同步葡萄糖抑制培养物中观察到的。我们的研究结果支持的概念，cAMP起着重要的作用，无论是在单个细胞和细胞周期相关的振荡在连续培养中，分别介导能量代谢和细胞周期进程的整合。有证据表明，在细胞周期的细胞内cAMP的动态行为调制取决于营养供应。这些研究结果的影响，cAMP在调节细胞周期进程和能量代谢的作用进行了讨论。版权所有（C）2003约翰威利父子有限公司。
We have investigated the role of 3',5'-cyclic-adenosine-monophosphate (cAMP) in mediating the coupling between energy metabolism and cell cycle progression in both synchronous cultures and oscillating continuous cultures of Saccharomyces cerevisiae. For the first time, a peak in intracellular cAMP was shown to precede the observed breakdown of trehalose and glycogen during cell cycle-related oscillations. Measurements in synchronous cultures demonstrated that this peak can be associated with the cell cycle dynamics of cAMP under conditions of glucose-limited growth, which was found to differ significantly from that observed in synchronous glucose-repressed cultures. Our results support the notion that cAMP plays a major role in mediating the integration of energy metabolism and cell cycle progression, both in the single cell and during cell cycle-related oscillations in continuous culture, respectively. Evidence is presented that the dynamic behaviour of intracellular cAMP during the cell cycle is modulated depending on nutrient supply. The implications of these findings regarding the role of cAMP in regulating cell cycle progression and energy metabolism are discussed. Copyright (C) 2003 John Wiley Sons, Ltd.