Quantitative determination of eugenol from Cinnamomum tamala nees and eberm.: Leaf powder and polyherbal formulation using reverse phase liquid chromatography
Quantitative determination of eugenol from Cinnamomum tamala nees and eberm.: Leaf powder and polyherbal formulation using reverse phase liquid chromatography
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DOI：
10.1365/s10337-005-0527-6
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发表时间：
2005-05-01
期刊：
影响因子：
1.7
通讯作者：
Patel, PH
中科院分区：
文献类型：
--
作者：
Dighe, VV;Gursale, AA;Patel, PH
建立了一种简便、快速、精确的反相高效液相色谱法定量测定番茄香樟叶干粉及其复方提取物中丁香酚的方法。色谱分析采用Zorbox C-18色谱柱（150 mm × 4.6 mm, 5 pm），流动相为水、乙腈和甲醇的混合物，体积比为50:40:10，流速为1.0 mL min（-1）。用紫外可见检测器在210 nm处进行定量。丁香酚在0.20 ~ 3.0 μ g mL（-1）范围内线性良好。
A simple, rapid and precise reverse-phase high performance liquid chromatographic method has been developed for the quantitative determination of eugenol from the extract of dried powder of Cinnamomum tomala leaves and its polyherbal formulation. Chromatographic analysis was carried out on Zorbox C-18 column (150 mm x 4.6 mm, 5 pm) with a mobile phase of mixture of water, acetonitrile and methanol in the volume ratio of 50:40:10, at a flow rate of 1.0 mL min(-1). Quantitation was performed using a UV-visible detector at 210 nm. Good linearity was obtained over the ranges of 0.20-3.0 mu g mL(-1) for eugenol.