Polysialylation takes place in granulosa cells during apoptotic processes of atretic tertiary follicles
Polysialylation takes place in granulosa cells during apoptotic processes of atretic tertiary follicles
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DOI：
10.1111/febs.13519
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发表时间：
2015-12-01
期刊：
影响因子：
5.4
通讯作者：
Galuska, Sebastian P.
中科院分区：
文献类型：
--
作者：
Kaese, Miriam;Galuska, Christina E.;Galuska, Sebastian P.
在神经系统中，聚唾液酸（polySia）已知参与几种细胞过程，如细胞-细胞相互作用的调节。这种高度带负电荷的糖部分主要作为神经细胞粘附分子（NCAM）的翻译后修饰存在。20多年前，在卵巢中检测到不同糖基化形式的NCAM。然而，NCAM的确切亚型及其生物学功能仍然未知。我们的分析表明，猫三级卵泡的颗粒细胞表达NCAM-140的多唾液酸化形式。出乎意料的是，polySia仅在闭锁卵泡的颗粒层中表达，而在健康卵泡中不表达。相比之下，健康卵泡的颗粒细胞膜上仅存在未聚唾液酸化形式的NCAM。为了研究polySia在猫卵泡中的可能细胞功能，使用原代颗粒细胞培养模型。有趣的是，polySia的损失导致细胞凋亡的显著抑制，表明polySia参与颗粒细胞的闭锁过程。因此，polySia可能不仅直接影响再生过程，例如在神经元系统中，而且还影响细胞凋亡。
In the neuronal system, polysialic acid (polySia) is known to be involved in several cellular processes such as the modulation of cell-cell interactions. This highly negatively-charged sugar moiety is mainly present as a post-translational modification of the neural cell adhesion molecule (NCAM). More than 20 years ago, differently glycosylated forms of NCAM were detected in the ovaries. However, the exact isoform of NCAM, as well as its biological function, remained unknown. Our analysis revealed that granulosa cells of feline tertiary follicles express the polysialylated form of NCAM-140. Unexpectedly, polySia was only expressed in the granulosa layers of atretic follicles and not of healthy follicles. By contrast, only the un-polysialylated form of NCAM was present on the membrane of granulosa cells of healthy follicles. To study a possible cellular function of polySia in feline follicles, a primary granulosa cell culture model was used. Interestingly, loss of polySia leads to a significant inhibition of apoptosis, demonstrating that polySia is involved during atretic processes in granulosa cells. Thus, polySia might not only directly influence regeneration processes as shown, for example, in the neuronal system, but also apoptosis.