Colocalization of the viral interleukin-6 with latent nuclear antigen-1 of human herpesvirus-8 in endothelial spindle cells of Kaposi's sarcoma and lymphoid cells of multicentric Castleman's disease
Colocalization of the viral interleukin-6 with latent nuclear antigen-1 of human herpesvirus-8 in endothelial spindle cells of Kaposi's sarcoma and lymphoid cells of multicentric Castleman's disease
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DOI：
10.1053/hupa.2001.21131
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Delsol, G
中科院分区：
文献类型：
--
作者：
Brousset, P;Cesarman, E;Delsol, G
人疱疹病毒-8（HHN-8）也称为拉波西肉瘤相关疱疹病毒，感染卡波西肉瘤（KS）中的梭形细胞和多中心Castleman病（MCD）中的淋巴细胞。在KS细胞中，HHV-8主要是潜伏的，表达潜伏核抗原-1（LNA-1），而在MCD中，溶血抗原和潜伏抗原均由淋巴细胞产生。我们通过免疫组化标记表明，在KS病毒白细胞介素-6（vIL-6）在罕见的梭形细胞中表达，而在MCD中，vIL-6可在淋巴滤泡周围的淋巴细胞中检测到，但也在滤泡树突状网状细胞网络内。抗IL-6抗体对凋亡小体的染色表明淋巴样细胞亚群中实现了完整的溶解周期。有趣的是，在MCD中，一些区域含有基于LNA-1表达的HHN-8潜伏感染的血管梭形细胞。这一发现可能意味着，在MCD中，血管和淋巴细胞增殖的病毒感染的反应。在MCD和KS中用抗LNA-1和抗vIL-6进行双重免疫染色，鉴定了2个HHV-8感染的亚群（血管和淋巴）细胞，一些细胞仅表达LNA-1，另一些细胞共表达vIL-6和LNA-1。这表明体内vIL-6和LNA-1蛋白表达的调节随细胞类型而变化。此外，在MCD中检测到感染的内皮细胞可能表明这些细胞属于HHV-8的储库。Copyright（C）2001 by W.B.桑德斯公司
Human herpesvirus-8 (HHN-8) also called Raposi's sarcoma-associated herpesvirus infects spindle cells in Kaposi's sarcoma (KS) and lymphoid cells in multicentric Castleman's disease (MCD). In KS cells, HHV-8 is mainly latent with the expression of latent nuclear antigen-1 (LNA-1), whereas in MCD both lyric and latent antigens are produced by lymphoid cells. We show by immunohistochemical labeling that in KS viral interleukin-6 (vIL-6) is expressed in rare spindle cells, whereas in MCD, vIL-6 is detectable in lymphoid cells around lymphoid follicles but also within the follicular dendritic reticulum cell network. The staining of apoptotic bodies with anti-IL-6 antibody suggests the achievement of a complete lytic cycle in a subset of lymphoid cells. Interestingly, in MCD, some areas contained vascular spindle cells latently infected by HHN-8 on the basis of LNA-1 expression. This finding might imply that in MCD, both vascular and lymphoid cells proliferate in response to the viral infection. Double immunostaining with anti-LNA-1 and anti-vIL-6 in MCD and KS identifies 2 subsets of HHV-8-infected (vascular and lymphoid) cells, some with exclusive expression of LNA-1 and some with coexpression of vIL-6 and LNA-1. This suggests that in vivo the regulation of the expression vIL-6 and LNA-1 protein varies with the cell type. In addition, the detection of infected endothelial cells in MCD may indicate that these cells belong to the reservoir for HHV-8. Copyright (C) 2001 by W.B. Saunders Company.