Peptidoglycan and peptidoglycan-specific ThI cells in psoriatic skin lesions
Peptidoglycan and peptidoglycan-specific ThI cells in psoriatic skin lesions
复制标题
银屑病皮损中的肽聚糖和肽聚糖特异性 ThI 细胞
DOI：
10.1002/path.1954
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发表时间：
2006-06-01
影响因子：
7.3
通讯作者：
Fry, L.
中科院分区：
文献类型：
--
作者：
Baker, B. S.;Laman, J. D.;Fry, L.
We have previously demonstrated, in psoriatic skin lesions, the presence of a subset of dermal CD4(+) T cells that produce interferon-gamma (IFN-gamma) in response to a mixture of cell wall proteins extracted from group A streptococci. However, the identity of the antigen(s) involved is unknown. To investigate the hypothesis that peptidoglycan (PG), the major constituent of the streptococcal cell wall, acts as a T cell activator in psoriasis, we performed in situ analysis to detect antigen-presenting cells containing PG in lesional versus non-lesional skin, and determined proliferation and IFN-gamma responses of lesional skin T cells. Increased numbers of PG-containing cells were detected in the dermal papillae and cellular infiltrates of guttate and chronic plaque skin lesions compared with normal and non-lesional psoriatic skin. A varying proportion of these were CD68(+) macrophages, but the remaining cells did not double stain for either Langerhans' or dendritic cell markers. Psoriatic dermal streptococcal-specific CD4(+) T cell lines proliferated and produced IFN-gamma in a self HLA-DR allele-restricted manner in response to streptococcal PG, excluding mitogenic or superantigenic stimulation, but were unresponsive to staphylococcal PG. Similarly, psoriatic staphylococcus-specific T cell lines recognized staphylococcal, but not streptococcal, PG by IFN-gamma production. The presence of PG-containing macrophages in close association with PG-specific CD4(+) T cells in lesional skin suggests that PG may be responsible, at least in part, for T cell activation in psoriasis. Copyright (c) 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
