Biodegradation of kraft lignin by a bacterial strain Comamonas sp B-9 isolated from eroded bamboo slips
Biodegradation of kraft lignin by a bacterial strain Comamonas sp B-9 isolated from eroded bamboo slips
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从受侵蚀的竹简中分离出的丛毛单胞菌 B-9 菌株对硫酸盐木质素的生物降解
DOI：
10.1111/j.1365-2672.2012.05275.x
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发表时间：
2012-05-01
影响因子：
4
通讯作者：
Zhang, H.
中科院分区：
文献类型：
--
作者：
Chen, Y. H.;Chai, L. Y.;Zhang, H.
目的：为获得单细胞菌Comamonas sp. B-9降解木质素的证据。方法与结果：将Comamonas sp. B-9接种于pH 7.0的硫酸盐木质素-无机盐培养基（KL-MSM）中，30 ℃培养7 d。细菌生长、化学需氧量（COD）降低、木质素降解酶的分泌和低分子量化合物的产生表明，丛毛单胞菌B-9能够降解硫酸盐木质素（KL）。KL-MSM中的COD在孵育7天后降低了32%。锰过氧化物酶（MnP）和漆酶（Lac）活性分别在培养第4天和第6天达到最大值，分别为29032 Ul（-1）和1250 Ul（-1）。GC-MS分析结果表明，丛毛单胞菌B-9在降解KL的过程中生成了大量的低分子化合物，如乙醇、3，5-二甲基苯甲醛和苯乙醇。结论：本研究证实了丛毛单胞菌B-9能够以KL为唯一碳源，将KL降解为低分子化合物。丛毛单胞菌B-9可用于生物技术应用中木质素和木质素衍生的芳族化合物的利用和生物转化。同时，利用丛毛单胞菌B-9处理制浆造纸废水是一项有意义的工作。
Aims: The aim was to obtain evidences for lignin degradation by unicellular bacterium Comamonas sp. B-9.Methods and Results: Comamonas sp. B-9 was inoculated into kraft lignin-mineral salt medium (KL-MSM) at pH 7.0 and 30 degrees C for 7 days of incubation. The bacterial growth, chemical oxygen demand (COD) reduction, secretion of ligninolytic enzymes and productions of low-molecular-weight compounds revealed that Comamonas sp. B-9 was able to degrade kraft lignin (KL). COD in KL-MSM reduced by 32% after 7 days of incubation. The maximum activities of manganese peroxidase (MnP) of 2903 2 U l(-1) and laccase (Lac) of 1250 U l(-1) were observed at 4th and 6th day, respectively. The low-molecular-weight compounds such as ethanediol, 3, 5-dimethyl-benzaldehyde and phenethyl alcohol were formed in the degradation of KL by Comamonas sp. B-9 based on GC-MS analysis.Conclusions: This study confirmed that Comamonas sp. B-9 could utilize KL as a sole carbon source and degrade KL to low-molecular-weight compounds.Significance and Impact of the Study: Comamonas sp. B-9 may be useful in the utilization and bioconversion of lignin and lignin-derived aromatic compounds in biotechnological applications. Meanwhile, using Comamonas sp. B-9 in treatment of wastewater in pulp and paper industry is a meaningful work.