Viral metagenomics analysis of feces from coronary heart disease patients reveals the genetic diversity of the Microviridae
Viral metagenomics analysis of feces from coronary heart disease patients reveals the genetic diversity of the Microviridae
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DOI：
10.1007/s12250-016-3896-0
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发表时间：
2017-04-01
影响因子：
5.5
通讯作者：
Cui, Li
中科院分区：
文献类型：
--
作者：
Guo, Lianghua;Hua, Xiuguo;Cui, Li
最近的研究表明，ssDNA 病毒科微病毒科的成员在多种环境中发挥着重要作用，因为它们被发现在人类肠道中占据主导地位。本研究的目的是分析冠心病（CHD）患者肠道病毒组的总体组成，并试图发现人类肠道病毒组与冠心病之间的潜在联系。采用病毒宏基因组学方法检测从先心病住院患者和作为对照的健康人收集的粪便样本中的病毒序列。我们对这些先心病患者和对照组的病毒组组成进行了分析。我们的数据表明，病毒组的组成可能与日常生活习惯和冠心病的药物治疗有关。 Virgaviridae 和 Microviridae 是在 CHD 患者肠道病毒组中发现的两种主要病毒类型。根据系统发育分析，发现了属于微病毒科的 14 种不同病毒，其中 12 种属于 Gokushovirinae 亚科，而其余两种病毒株可能代表微病毒科内的两个新亚科。此外，这些病毒的基因组组织也已被表征。
Recent studies have declared that members of the ssDNA virus family Microviridae play an important role in multiple environments, as they have been found taking a dominant position in the human gut. The aim of this study was to analyze the overall composition of the gut virome in coronary heart disease (CHD) patients, and try to discover the potential link between the human gut virome and CHD. Viral metagenomics methods were performed to detect the viral sequences in fecal samples collected from CHD inpatients and healthy persons as controls. We present the analysis of the virome composition in these CHD patients and controls. Our data shows that the virome composition may be linked to daily living habits and the medical therapy of CHD. Virgaviridae and Microviridae were the two dominant types of viruses found in the enteric virome of CHD patients. Fourteen divergent viruses belonging to the family Microviridae were found, twelve of which were grouped into the subfamily Gokushovirinae, while the remaining two strains might represent two new subfamilies within Microviridae, according to the phylogenetic analysis. In addition, the genomic organization of these viruses has been characterized.