The in vitro replication of DNA containing the SV40 origin.
The in vitro replication of DNA containing the SV40 origin.
复制标题
含有 SV40 起点的 DNA 的体外复制。
DOI：
10.1016/s0021-9258(17)44708-9
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Suk
中科院分区：
文献类型：
--
作者：
J. Hurwitz;F. Dean;Ann D. Kwong;Suk
SV40 基因组在小型（5243 个碱基对）共价闭合双链 DNA 基因组内包含单个 DNA 复制起点，并被组织成包含源自宿主细胞的组蛋白的微型染色体。除病毒编码的大T抗原外，SV40复制所需的所有蛋白质均由宿主细胞提供。因此，SV40 DNA 复制的许多必需宿主蛋白可能在细胞 DNA 复制中发挥类似的作用。 Li 和 Kelly 建立的体外 SV40 DNA 复制系统 (1) 对于表征在 SV40 复制中发挥作用的宿主蛋白至关重要。本综述将重点关注 SV40 DNA 复制的途径以及参与 DNA 合成反应的蛋白质。最近还出现了有关真核 DNA 复制的其他评论 (2-5)。
The SV40 genome contains a single origin of DNA replication within a small (5243 base pairs) covalently closed double-stranded DNA genome and is organized into minichromosomes containing histones derived from the host cell. With the exception of the virus-encoded large T antigen, all the proteins required for SV40 replication are supplied by the host cell. Thus, many of the essential host proteins for SV40 DNA replication probably play a similar role in cellular DNA replication. The establishment of an in vitro SV40 DNA replication system by Li and Kelly (1) has been pivotal in allowing the characterization of the host proteins which play a role in SV40 replication. This review will focus on the pathway of SV40 DNA replication and the proteins involved in the DNA synthesis reactions. Other reviews of eukaryotic DNA replication have also appeared recently (2-5).