Self-assembled hybrid nanoparticles for cancer-specific multimodal imaging
Self-assembled hybrid nanoparticles for cancer-specific multimodal imaging
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DOI：
10.1021/ja073062z
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发表时间：
2007-07-25
影响因子：
15
通讯作者：
Lin, Wenbin
中科院分区：
文献类型：
--
作者：
Kim, Jason S.;Rieter, William J.;Lin, Wenbin
一层一层（LbL）的沉积策略用于制备多功能纳米粒子（MFNP）与多模态成像能力。交替处理含有发光[Ru（bpy）（3）]Cl-2核的杂化二氧化硅纳米颗粒（NP 0）和具有阳离子Gd（III）-DOTA低聚物1和阴离子聚（苯乙烯磺酸盐）（PSS）的Gd-（正丙基）二亚乙基三胺四乙酸盐（Gd-DTTA）络合物的阴离子单层涂层导致1和PSS的多层膜通过静电相互作用沉积。这种LbL沉积技术提供了一个极好的策略，用于组装具有嵌入式发光体和非常高的MR弛豫率的混合纳米颗粒。PSS封端的多层纳米颗粒可以在生理条件下通过静电相互作用用携带正电荷的靶向肽非共价官能化，以产生用于HT-29人结肠癌细胞的光学和MR成像的癌症特异性MFNP。这种方法的通用性应该允许设计可以特异性靶向广泛的病变细胞的成像和/或治疗MFNP。
A layer-by-layer (LbL) polyelectrolyte deposition strategy is used to prepare multifunctional nanoparticles (MFNPs) with multimodal imaging capabilities. Alternating treatment of hybrid silica nanoparticles (NP0) containing a luminescent [Ru(bpy)(3)]Cl-2 core and anionic monolayer coating of the Gd-(siloxylpropyl)diethylenetriamine tetraacetate (Gd-DTTA) complex with cationic Gd(III)-DOTA oligomer 1 and anionic poly(styrene sulfonate) (PSS) led to the deposition of multilayers of 1 and PSS via electrostatic interactions. This LbL deposition technique offers a superb strategy for the assembly of hybrid nanoparticles with imbedded luminophores and very high MR relaxivities. The PSS-terminated multilayered nanoparticles can be noncovalently functionalized with targeting peptides that carry positive charges under physiological conditions via electrostatic interactions to lead to cancer-specific MFNPs for optical and MR imaging of HT-29 human colon cancer cells. The generality of this approach should allow the design of imaging and/or therapeutic MFNPs that can specifically target a wide range of diseased cells.