Frequency of occurrence of pneumonia associated agents and their correlation with clinical and pathological-anatomical findings in pigs
Frequency of occurrence of pneumonia associated agents and their correlation with clinical and pathological-anatomical findings in pigs
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DOI：
10.2376/0005-9366-120-483
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发表时间：
2007-11-01
影响因子：
0.4
通讯作者：
Heinritzi, Karl
中科院分区：
文献类型：
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作者：
Palzer, Andreas;Ritzmann, Mathias;Heinritzi, Karl
本研究的目的是研究猪肺炎和支气管肺炎发生过程中不同致病微生物的发生率和影响。对339头猪支气管肺泡灌洗液（BALF）进行了分子生物学和培养学检测。评价考虑了以下病原体：支原体（M.）hypopteriosma，Mycoplosma（M.）猪鼻病毒、PRRSV（US型）、PRRSV（EU型）、PCV-2、A型流感病毒、α-溶血性链球菌、β-溶血性链球菌、巴氏杆菌（P.）多杀性、博德特氏菌（B.）支气管败血症、Hoemophilus（H.）副猪放线杆菌（A.）胸膜肺炎随后对168头猪进行了病理解剖学检查。病理解剖检查评估可能的间质性肺炎，卡他性化脓性支气管肺炎和胸膜炎。（x-溶血性链球菌、PCV-2、H. parosuis，M. hyorhinis、猪鼻霉M.下丘脑B、B.支气管败血症、PRRSV（US型）、多杀性巴斯德菌、PRRSV（EU型）和流感病毒A型在BALF中的发病率明显下降。本文报道了甲型溶血性链球菌、M.猪流感、多杀性巴氏杆菌和B. B.支气管败血症与确定的临床评估评分和病理解剖学检查评分显著相关。M.猪鼻病毒和A型流感病毒仅与临床表现的严重程度相关，而PCV-2和PRRSV（US型）与病理解剖学变化的频率相关。临床评分越高，确定具有五种或五种以上不同病原体的动物数量越多。在一只动物的BALF中分离出的不同致病因子越多，平均病理解剖学检查评分越高。对于肺炎的诊断，特别是当分析兼性病原体或继发性病原体时，只有结合临床和病理学评估才可能对分析结果进行有用的解释。
The aim of this study was to investigate the incidence and influence of different causative organisms involved in the development of pneumonia and bronchopneumonia in pigs. Bronchoalveolar lavage fluid (BALF) from 339 pigs was examined molecular-biologically and culturally. The evaluation considered the following pathogens: Mycoplasma (M.) hyopneumoniae, Mycoplosma (M.) hyorhinis, PRRSV (US-Type), PRRSV (EU-Type), PCV-2, Influenzavirus Type A, alpha-haemolytic Streptococci, P-haemolytic Streptococci, Pasteurella (P.) multocida, Bordetella (B.) bronchiseptica, Hoemophilus (H.) parasuis and Actinobacillus (A.) pleuropneumoniae. This was followed by a pathological-anatomical examination in 168 pigs. Pathologicalanatomical examination was evaluated for possible interstitial pneumonia, catarrhal-purulent bronchopneumonia and pleuritis.(x-haemolytic Streptococci, PCV-2, H. parosuis, M. hyorhinis, M. hyopneumoniae, B. bronchiseptica, PRRSV (US-Type), P multocida, PRRSV (EU-Type) and Influenzavirus Type A were evident in descending incidence in the BALF.A.pleuropneumoniae were only isolated culturally in two cases in the BALF. The frequency and semiquantitative detection rate in the bacteriological culture of a-haemolytic Steptococci, M. hyopneumoniae, P.multocida and B. bronchiseptica correlated significantly with the ascertained clinical evaluation score and the pathological-anatomical examination score. M. hyorhinis and Influenzavirus Type A only correlated with the severity degree of clinical appearance, while PCV-2 and PRRSV (US-Type) correlated with the frequency of pathological-anatomical changes. The higher the clinical score, the higher was the number of animals ascertained with five or more different pathogens. The more different causal agents were isolated in the BALF of one animal, the higher was the average pathological-anatomical examination score. For the diagnosis of pneumonia, especially when analysing facultative pathogens or secondary pathogens, a useful interpretation of analysis results is only possible in connection with a clinical and pathological evaluation.