Water deficit triggers phospholipase D activity in the resurrection plant Craterostigma plantagineum
Water deficit triggers phospholipase D activity in the resurrection plant Craterostigma plantagineum
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DOI：
10.1105/tpc.12.1.111
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发表时间：
2000-01-01
期刊：
影响因子：
11.6
通讯作者：
Bartels, D
中科院分区：
文献类型：
--
作者：
Frank, W;Munnik, T;Bartels, D
磷脂在动物细胞的许多信号通路中起着重要作用。磷脂切割酶如磷脂酶C、D （PLD）和A的激活可触发信号级联反应(2)。它们的活动导致第二信使的形成和初始信号的放大。在这项研究中，我们提供了实验证据，证明PLD参与了复活植物车前草（craterero柱头plantagineum）脱水的早期事件。PLD蛋白的酶活性在脱水开始后几分钟内被激活，尽管它不是由脱落酸诱导的，但PLD活性确实在乳腺炎蛋白的作用下增加，这表明异三聚体G蛋白在PLD调节中起作用。分离到两个编码pld的cDNA克隆：CpPLD-1和CpPLD-2。CpPLD-1是组成型表达，而CpPLD-2是由脱水和脱落酸诱导表达的。免疫学研究揭示了脱水后PLD蛋白亚细胞定位的变化。综上所述，酶活性以及转录物和蛋白质分布的数据使我们能够提出PLD在导致金车前草耐干燥性的事件中的作用。
Phospholipids play an important role in many signaling pathways in animal cells. Signaling cascades are triggered by the activation of phospholipid cleaving enzymes such as phospholipases C, D (PLD), and A(2). Their activities result in the formation of second messengers and amplification of the initial signal. In this study, we provide experimental evidence that PLD is involved in the early events of dehydration in the resurrection plant Craterostigma plantagineum. The enzymatic activity of the PLD protein was activated within minutes after the onset of dehydration, and although it was not inducible by abscisic acid, PLD activity did increase in response to mastoparan, which suggests a role for heterotrimeric G proteins in PLD regulation. Two cDNA clones encoding PLDs, CpPLD-1 and CpPLD-2, were isolated. The CpPLD-1 transcript was constitutively expressed, whereas CpPLD-2 was induced by dehydration and abscisic acid. Immunological studies revealed changes in the subcellular localization of the PLD protein in response to dehydration. Taken together, the data on enzymatic activity as well as transcript and protein distributions allowed us to propose a role for PLD in the events leading to desiccation tolerance in C. plantagineum.