E1-Ngn2/Cre is a new line for regional activation of cre recombinase in the developing CNS
E1-Ngn2/Cre is a new line for regional activation of cre recombinase in the developing CNS
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DOI：
10.1002/gene.20081
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发表时间：
2004-12-01
期刊：
影响因子：
1.5
通讯作者：
Stoykova, A
中科院分区：
文献类型：
--
作者：
Berger, J;Eckert, S;Stoykova, A
我们生成了名为 E1-Ngn2/Cre 的转基因小鼠系，其在 Ngn2 基因的 E1 增强子元件的控制下表达 Cre 重组酶和 GFP（Scardigli 等人：Neuron 31：203-217，2001）。 Cre 重组酶活性和 GFP 荧光与报道的由 E1-Ngn2 增强子控制的表达模式一致。在神经管心室区的祖细胞结构域 p1 和 p2 以及前顶盖、背侧和腹侧丘脑、中脑被盖和后脑的不同区域中检测到重组。在发育中的皮质中，Cre 重组酶活性仅限于主要位于大脑皮层腹侧和外侧区域的祖细胞亚群。因此，E1-Ngn2/Cre 小鼠品系为发育中的 CNS 中的区域特异性条件诱变提供了一种极好的新工具。 (C) 2004 Wiley-Liss, Inc.
We generated a transgenic mouse line named E1-Ngn2/Cre that expresses Cre recombinase and GFP under the control of the E1 enhancer element of the gene Ngn2 (Scardigli et al.: Neuron 31:203-217, 2001). Cre-recombinase activity and GFP fluorescence are consistent with the reported expression pattern controlled by the E1-Ngn2 enhancer. Recombination was detected in the progenitor domains p1 and p2 in the ventricular zone of the neural tube and in distinct domains of the pretectum, the dorsal and ventral thalamus, the tegmentum of the mesencephalon, and the hindbrain. In the developing cortex, Cre-recombinase activity is confined to a sub-population of progenitors predominantly in the region of the ventral and lateral pallium. The E1-Ngn2/Cre mouse line thus provides an excellent novel tool for a region-specific conditional mutagenesis in the developing CNS. (C) 2004 Wiley-Liss, Inc.