Effects of harvesting cell density, medium depth and environmental factors on biomass and lipid productivities of Chlorella vulgaris grown in swine wastewater
Effects of harvesting cell density, medium depth and environmental factors on biomass and lipid productivities of Chlorella vulgaris grown in swine wastewater
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DOI：
10.1016/j.ces.2016.06.025
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发表时间：
2016-10
影响因子：
4.7
通讯作者：
H. Amini;Lijun Wang;A. Shahbazi
中科院分区：
文献类型：
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作者：
H. Amini;Lijun Wang;A. Shahbazi
建立了回归模型来确定小球藻的生长速率受温度、光照强度和 pH 值等环境因素的影响。通过实验确定了普通念珠菌生长的最佳环境条件为光强度为240 μE m− 2 s− 1、24°C、pH为7.4。在最佳环境条件下，普通念珠菌在含有 102 mg N/l 和 76 mg P/l 的猪废水中的生长率为 0.160 g/l/天，而在含有 100 mg N/l 和 53 mg P/l 的改良 Bold 培养基上的生长率为 0.191 g/l/天。回归模型进一步与光热传递模型相结合，以估计在北卡罗来纳州每年的天气条件下，在具有不同介质深度和收获细胞密度的开放式水道池塘（ORP）中，在猪废水中生长的普通念珠菌的生物量生产力。在 20 cm 介质深度处，最高生长速率为 0.162 g/l/天，这是在 8 月份收获细胞密度 0.1 g/l、24°C 和 1350 μE m− 2 s− 1 太阳辐照度下获得的。如果培养基深度增加至 30 cm，则 6 月份在 23°C、1500 μE m− 2 s− 1 条件下获得的 0.1 g/l 收获细胞密度下的最高生长率为 0.156 g/l/天。如果收获细胞密度增加至 0.4 克/升，则 20 厘米和 30 厘米培养基深度的最高生长速率分别显着下降至 0.033 和 0.02 克/升/天。在收获细胞密度为 0.1 克/升时，30 厘米和 20 厘米中等深度的藻类年生产力分别为 80 吨/公顷和 59 吨/公顷。在养猪废水中生长的C. vulgaris的平均脂质含量为25%时，在30厘米和20厘米中等深度处，最高脂质产量分别为20和14.75吨/公顷/年。
A regression model was developed to determine the growth rate of Chlorella vulgaris that is affected by the environmental factors of temperature, light intensity and pH value. The optimum environmental condition for growing C. vulgaris was experimentally determined at light intensity of 240 μ E m− 2 s− 1, 24° C and pH of 7.4. At the optimum environmental condition, the growth rate of C. vulgaris in swine wastewater with 102 mg N/l and 76 mg P/l was 0.160 g/l/day, compared to 0.191 g/l/day for its growth on a modified Bold's medium with 100 mg N/l and 53 mg P/l. The regression model was further integrated with a light and heat transfer model to estimate the biomass productivity of C. vulgaris grown on the swine wastewater in an open raceway pond (ORP) with different medium depths and harvesting cell densities under the weather condition in North Carolina yearly around. At 20 cm medium depth, the highest growth rate was 0.162 g/l/day, which was obtained at 0.1 g/l harvesting cell density, 24° C and 1350 μ E m− 2 s− 1 solar irradiance in August. If the medium depth increased to 30 cm, the highest growth rate at 0.1 g/l harvesting cell density was 0.156 g/l/day, which was obtained at 23° C and 1500 μ E m− 2 s− 1 in June. If the harvesting cell density increased to 0.4 g/l, the highest growth rate decreased significantly to 0.033 and 0.02 g/l/day for 20 cm and 30 cm medium depths, respectively. At 0.1 g/l harvesting cell density, the yearly algal productivity was 80 and 59 t/ha at 30 cm and 20 cm medium depths, respectively. At the average 25% lipid content of C. vulgaris grown in swine wastewater, the highest lipid yield was 20 and 14.75 t/ha/year at 30 cm and 20 cm medium depths, respectively.