過酸化水素の存在における4-(2-ピリジルアゾ)-レゾルシノールによるクロム(III)の吸光光度法
過酸化水素の存在における4-(2-ピリジルアゾ)-レゾルシノールによるクロム(III)の吸光光度法
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在过氧化氢存在下用 4-(2-吡啶偶氮)-间苯二酚分光光度法测定铬(III)
DOI：
10.2116/bunsekikagaku.27.7_460
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发表时间：
1978
期刊：
影响因子：
0.2
通讯作者：
裕一 高野
中科院分区：
文献类型：
--
作者：
勝巳 山本;裕一 高野
我们发现，当铬（III）的氧化态在过氧化氢的存在下转变为（IV）或（V）时，铬（III）与络合剂的反应性被加速。因此，我们将这一发现应用于铬（III）与4-（2-吡啶偶氮）-间苯二酚（PAR）的反应，获得了更灵敏、更简便的测定铬（III）的方法。移取一定量的铬（III）、2 ml 5.00 × 10-3 M PAR、2 ml 2 M乙酸-乙酸钠缓冲液和3 ml 1 M过氧化氢，置于100 ml带塞子的锥形瓶中。通过加入再蒸馏水使最终体积达到50 ml。将溶液在恒温控制的水浴中在40°C下加热20分钟。冷却至室温后，将一部分溶液转移至池中。在530 nm处测量吸光度。通过上述程序获得铬（III）浓度范围为（0.100.52）μg ml-1的校准曲线。在530 nm处的摩尔吸光系数为5.1 × 1041 mol-1 cm-1。方法灵敏度高，操作简便。
We found that the reactivity of chromium (III) with complexing agents was accelerated when the oxidation state of chromium (III) was turned to change to (IV) or (V) in the presence of hydrogen peroxide. Consequentry, we applied the finding to the reaction of chromium (III) and 4-(2-pyridylazo)-resorcinol (PAR) to obtain more sensitive and simple method for the determination of chromium (III). A given quantity of chromium (III), 2 ml of 5.00 × 10-3 M PAR, 2 ml of 2 M acetic acid-sodium acetate buffer solution, and 3 ml of 1 M hydrogen peroxide were pipetted into a 100 ml Erlenmeyer flask fitted with a plug. The final volume was brought to 50 ml by the addition of redistilled water. The solution was warmed for 20 min at 40°C in a thermostatically-controlled water bath. After cooling to room temperature, a portion of the solution was then transferred into a cell. The measurements of absorbance were made at 530 nm. The calibration curve was obtained by the above procedure for the concentration range of (0.100.52) μg ml-1 of chromium (III). The molar absorptivity at 530 nm was calculated to be 5.1 × 104 1 mol-1 cm-1. The method is very sensitive and simple.