CONCENTRATION-DEPENDENT EFFECTS OF DENTIN PHOSPHORYN IN THE REGULATION OF INVITRO HYDROXYAPATITE FORMATION AND GROWTH
CONCENTRATION-DEPENDENT EFFECTS OF DENTIN PHOSPHORYN IN THE REGULATION OF INVITRO HYDROXYAPATITE FORMATION AND GROWTH
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DOI：
10.1016/0169-6009(90)90015-8
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发表时间：
1990-10-01
期刊：
影响因子：
--
通讯作者：
VEIS, A
中科院分区：
文献类型：
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作者：
BOSKEY, AL;MARESCA, M;VEIS, A
在体外明胶凝胶扩散系统中研究了牙本质磷蛋白对羟基磷灰石形成和生长的影响。低浓度（0.010-1μg/ml）的磷磷灰石促进羟基磷灰石从头形成；在同一系统中较高浓度（100μg/ml）时，牙本质基质蛋白抑制羟基磷灰石生长。在溶液中也观察到类似的羟基磷灰石生长抑制作用。完整的磷酸蛋白对于抑制种子生长或促进矿化都不是必需的，因为甲酸降解的蛋白质相对有效。 7天时形成的沉淀物的透射电子显微镜显示，在存在和不存在磷酸化物的情况下，微晶尺寸分布没有显着差异。然而，在磷酰蛋白含量增加的情况下，形成的矿物簇的数量呈剂量依赖性减少，表明二次成核受到抑制。这些数据为牙本质磷蛋白在矿化过程中假定的“多功能”作用提供了支持。
The effect of dentin phosphophoryn on hydroxyapatite formation and growth was studied in an in vitro gelatin gel diffusion system. Phosphophoryn, in low concentrations (0.010-1 .mu.g/ml) promoted de novo hydroxyapatite formation; at a higher concentration (100 .mu.g/ml) in the same system, the dentin matrix protein inhibited hydroxyapatite growth. Similar inhibition of hydroxyapatite growth was seen in solution. The intact phosphophoryn was not essential for either inhibition of seeded growth or promotion of mineralization, since the formic acid degraded protein was comparably effective. Transmission electron microscopy of the precipitates formed at 7 days showed no significant differences in crystallite size distribution in the presence and absence of phosphophoryn. However there was a dose-dependent decrease in the number of mineral clusters formed in the presence of increasing amounts of phosphophoryn, suggesting inhibition of secondary nucleation. These data provide support for the postulated ''multifunctional'' role of the dentin phosphoprotein in the mineralization process.