Influence of experimental diabetes on the microcirculation of injured peripheral nerve - Functional and morphological aspects
Influence of experimental diabetes on the microcirculation of injured peripheral nerve - Functional and morphological aspects
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DOI：
10.2337/diabetes.51.7.2233
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发表时间：
2002-07-01
期刊：
影响因子：
7.7
通讯作者：
Zochodne, DW
中科院分区：
文献类型：
--
作者：
Kennedy, JM;Zochodne, DW
糖尿病轴突的再生在起病、分级和成熟方面都有延迟。周围神经的血管供应——神经血管的微血管病变可能导致局部环境对神经再生不利。我们用激光多普勒血流仪和微电极氢清除极谱法检测了长期（8个月）实验性链脲佐菌素糖尿病大鼠坐骨神经近端和远端断断的局部神经血流。然后，我们将这些发现联系起来，使用印度墨水制剂的体内灌注，通过勾勒出来自未固定神经切片的微血管的管腔。糖尿病患者和非糖尿病患者未损伤神经的基线神经血流量没有差异，血管数量、密度和面积没有改变。横断后，与糖尿病动物相比，非糖尿病动物近端残端神经的血流量增加更大，这与神经外血管的数量、密度和口径有关。非糖尿病神经远端残端也发生充血，但糖尿病神经未发生充血。在这些残肢中，糖尿病大鼠血管数量减少，平均神经内膜血管面积减小。糖尿病周围神经损伤后神经血流上调失败或延迟可能会产生相对缺血的再生微环境。
Regeneration of diabetic axons has delays in onset, rated and maturation. It is possible that microangiopathy of vasa nervorum, the vascular supply of the peripheral nerve, may render an unfavorable local environment for nerve regeneration. We examined local nerve blood flow proximal and distal to sciatic nerve transection in rats with long-term (8 month) experimental streptozotocin diabetes using laser Doppler flowmetry and microelectrode hydrogen clearance polarography. We then correlated these findings, using in vivo perfusion of an India ink preparation, by outlining the lumens of microvessels from unfixed nerve sections. There were no differences in baseline nerve blood flow between diabetic and nondiabetic uninjured nerves, and vessel number, density, and area were unaltered. After transection, there were greater rises in blood flow in proximal stumps of nondiabetic nerves than in diabetic animals associated with a higher number, density, and caliber of epineurial vessels. Hyperemia also developed in distal stumps of nondiabetic nerves but did not develop in diabetic nerves. in these stumps, diabetic rats had reduced vessel numbers and smaller mean endoneurial vessel areas. Failed or delayed upregulation of nerve blood flow after peripheral nerve injury in diabetes may create a relatively ischemic regenerative microenvironment.