Effects of nitrogen catabolite repression and di-ammonium phosphate addition during wine fermentation by a commercial strain of S. cerevisiae
Effects of nitrogen catabolite repression and di-ammonium phosphate addition during wine fermentation by a commercial strain of S. cerevisiae
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DOI：
10.1007/s00253-011-3084-y
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发表时间：
2011-03-01
影响因子：
5
通讯作者：
Gardner, Richard C.
中科院分区：
文献类型：
--
作者：
Deed, Nathan K.;van Vuuren, Hennie J. J.;Gardner, Richard C.
在商业酿酒酵母背景M2中构建了两个在氮分解代谢阻遏(NCR)方面有缺陷的缺失突变体：一个是ure2突变体，一个是dal80gzf3双突变体。野生型和突变型菌株在添加和不添加磷酸二铵(DAP)的白苏维翁葡萄汁中发酵。Dal80gzf3双突变体表现出较长的发酵滞后期，但可被DAP(相当于300 mg/L的N)所抵消。NCR突变和DAP添加都不影响最终葡萄酒中挥发性硫醇的含量。对野生型和dal80gzf3双突变株在2%和70%糖发酵后的转录本进行了基因芯片分析。在之前确定的80个NCR调控基因中，只有13个基因在葡萄汁中发酵过程中上调。在DAP添加后，34个已知的NCR基因下调，其中17个甚至在NCR突变株中也下调。结果表明，NCR反应具有意想不到的复杂性，这可能反映了酵母菌株之间的差异，或者与标准有氧生长相比，酒精发酵过程中基因调控的差异。
Two deletion mutants expected to be defective in nitrogen catabolite repression (NCR) were constructed in a commercial wine yeast background M2: a ure2 mutant and a dal80 gzf3 double mutant. Wild-type and both mutant strains were fermented in Sauvignon Blanc grape juice with and without addition of di-ammonium phosphate (DAP). The dal80 gzf3 double mutant exhibited a long fermentative lag phase, which was offset by DAP addition (corresponding to 300 mg/L of N). Neither the NCR mutations nor DAP addition affected the content of volatile thiols in the final wine. Microarray analyses of transcripts in the wild-type and dal80 gzf3 double-mutant strains were performed after 2% and 70% sugars were fermented. Of 80 genes previously identified as NCR-regulated, only 13 were upregulated during fermentation of the dal80 gzf3 double-mutant strain in grape juice. Following DAP addition, 34 of the known NCR genes were downregulated, including 17 that were downregulated even in the NCR mutant strain. The results demonstrate an unexpected complexity of the NCR response that may reflect differences between strains of yeast or differences in gene regulation during alcoholic fermentation compared with standard aerobic growth.