Silefrin, a sodefrin-like pheromone in the abdominal gland of the sword-tailed newt, Cynops ensicauda
Silefrin, a sodefrin-like pheromone in the abdominal gland of the sword-tailed newt, Cynops ensicauda
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DOI：
10.1016/s0014-5793(00)01455-1
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发表时间：
2000-04-28
期刊：
影响因子：
3.5
通讯作者：
Kikuyama, S
中科院分区：
文献类型：
--
作者：
Yamamoto, K;Kawai, Y;Kikuyama, S
采用凝胶过滤色谱和反相高效液相色谱法从剑尾蝾螈雄性腹腺中分离纯化了一种类似Sodefrin的雌性引诱信息素。最终产物含有10个氨基酸残基，序列SILSKDAQLK，与其前体cDNA推导的序列一致。silefrin的序列与sodefrin bg的序列有两个不同的氨基酸残基，分别在第3位和第8位由Leu取代Pro，由Gln取代Leu。天然和合成silefrin发挥了同等效力的活动，吸引同种女性。(C)2000年欧洲生物化学学会联合会。
Sodefrin-like female-attracting pheromone was purified from the abdominal glands of male sword-tailed newts, Cynops ensicauda, by gel-filtration chromatography and reversed-phase high-performance liquid chromatography. The final product comprises 10 amino acid residues with the sequence SILSKDAQLK,which coincided with the sequence deduced from its precursor cDNA. This peptide was designated silefrin, The sequence of silefrin was different from that of sodefrin bg two amino acid residues, with substitutions Leu for Pro and Gin for Leu at positions 3 and 8, respectively. Both native and synthetic silefrin exerted an equipotent activity in attracting conspecific females. (C) 2000 Federation of European Biochemical Societies.