Microbial communities in contaminated sediments, associated with bioremediation of uranium to submicromolar levels
Microbial communities in contaminated sediments, associated with bioremediation of uranium to submicromolar levels
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DOI：
10.1128/aem.02308-07
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发表时间：
2008-06-01
影响因子：
4.4
通讯作者：
Tiedje, James A.
中科院分区：
文献类型：
--
作者：
Cardenas, Erick;Wu, Wei-Min;Tiedje, James A.
采用微生物计数、16 S rRNA基因克隆文库和化学分析方法，在美国能源部位于田纳西州橡树岭的一个高铀污染场地（高达60 mg/L和800 mg/kg固体）进行了一项长期实验（超过2年），以评估铀（VI）的原位生物还原和固定。生物还原是通过调节地面以上的地下水，然后通过每周向地下注入乙醇来原位刺激微生物、Fe（III）还原菌和硫酸盐还原菌的生长来实现的。经过近2年的间歇性注入乙醇，含水铀水平下降到低于美国环境保护署的饮用水和地下水最高污染水平（
Microbial enumeration, 16S rRNA gene clone libraries, and chemical analysis were used to evaluate the in situ biological reduction and immobilization of uranium(VI) in a long-term experiment (more than 2 years) conducted at a highly uranium-contaminated site (up to 60 mg/liter and 800 mg/kg solids) of the U.S. Department of Energy in Oak Ridge, TN. Bioreduction was achieved by conditioning groundwater above ground and then stimulating growth of denitrifying, Fe (III)-reducing, and sulfate-reducing bacteria in situ through weekly injection of ethanol into the subsurface. After nearly 2 years of intermittent injection of ethanol, aqueous U levels fell below the U.S. Environmental Protection Agency maximum contaminant level for drinking water and groundwater (