Feather pecking behavior in laying hens: Hypothalamic gene expression in birds performing and receiving pecks
Feather pecking behavior in laying hens: Hypothalamic gene expression in birds performing and receiving pecks
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DOI：
10.3382/ps.2010-00961
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发表时间：
2011-06-01
期刊：
影响因子：
4.4
通讯作者：
Keeling, L.
中科院分区：
文献类型：
--
作者：
Brunberg, E.;Jensen, P.;Keeling, L.
啄羽是蛋生产部门的一个福利和经济问题。目前用于减少FP的喙修剪方法也受到批评。目前的研究使用基因表达来探索这种行为背后的生物学机制，这可能会导致对原因的更好理解和减轻问题的工具。在商业农场中鉴别了执行和接受FP的白色来航母鸡以及中性对照鸡。将来自11只啄木鸟、10只受害者和10只对照的下丘脑RNA杂交到基因芯片鸡基因组阵列（Affyphidian Inc.，Santa Clara，CA）以比较不同组中的基因表达谱。10个基因对应的11个转录本在3组间差异显著（校正P <0.05）。这些成绩单中有8个不同的啄木鸟与对照组相比，1个上调的受害者与对照组相比，和6个不同的啄木鸟与受害者相比显着。此外，5个转录物显示出在啄木鸟-受害者比较中不同的趋势（调整的P <0.1）。一些差异表达基因的产物参与疾病，如肠道炎症和胰岛素抵抗，这与先前提出的FP是一种异常觅食行为的假设非常吻合。其他研究结果也可能支持FP与免疫机制有关的提议，并可能作为人类强迫症的动物模型。总之，这项研究提供了一个基因列表，可能有助于进一步研究FP背后的机制。
Feather pecking (FP) is a welfare and economic problem in the egg production sector. Beak trimming, the current method used to reduce FP, is also criticized. The present study used gene expression to explore the biological mechanisms underlying this behavior, which could lead to a greater understanding of the cause and a tool to mitigate the problem. White Leghorn hens performing and receiving FP, as well as neutral control birds, were identified on a commercial farm. Hypothalamic RNA from 11 peckers, 10 victims, and 10 controls was hybridized onto GeneChip Chicken Genome Arrays (Affymetrix Inc., Santa Clara, CA) to compare gene expression profiles in the different groups. Eleven transcripts corresponding to 10 genes differed significantly between the 3 groups (adjusted P < 0.05). Eight of these transcripts differed in the peckers compared with the controls, 1 was upregulated in the victims compared with the controls, and 6 differed significantly in the peckers compared with the victims. Additionally, 5 transcripts showed a trend (adjusted P < 0.1) to differ in the pecker-victim comparison. Some of the products of the differently expressed genes are involved in disorders, such as intestinal inflammation and insulin resistance, which fit well with the previously proposed hypothesis that FP is an abnormal foraging behavior. Other findings may also support the proposal that FP is linked to immune mechanisms and may serve as an animal model for obsessive compulsive disorder in humans. In conclusion, this study provides a gene list that may be useful in further research on the mechanisms behind FP.