Neuropeptide Y is expressed by osteocytes and can inhibit osteoblastic activity.
Neuropeptide Y is expressed by osteocytes and can inhibit osteoblastic activity.
复制标题
DOI：
10.1002/jcb.22294
复制
发表时间：
2009-10-15
影响因子：
4
通讯作者：
Kalajzic, Ivo
中科院分区：
文献类型：
--
作者：
Igwe, John C.;Jiag, Xi;Paic, France;Ma, Li;Adams, Douglas J.;Baldock, Paul A.;Pilbeam, Carol C.;Kalajzic, Ivo
骨细胞是骨基质中最丰富的成骨细胞系。它们对机械刺激作出反应，并参与调节蛋白的释放，从而调节其他骨细胞的活动。我们假设神经肽Y （NPY）是一种在骨形成中具有调节功能的神经递质，由骨细胞产生并影响成骨细胞活性。为了研究NPY在成骨细胞系细胞中的表达，我们利用转基因小鼠模型鉴定和分离成骨细胞和骨细胞群体。Col2.3GFP转基因在成骨细胞和骨细胞中有活性，而DMP1启动子在骨细胞中驱动绿色荧光蛋白（GFP）的表达。对新生儿颅骨分离细胞群体的RNA进行实时PCR分析显示，与成骨细胞相比，前骨细胞/骨细胞部分的NPY mRNA含量更高。NPY免疫染色证实NPY在骨细胞（DMP1GFP+）中强表达，在成骨细胞中低表达。此外，NPY受体Y1 mRNA在鼠腔和长骨以及初代颅骨成骨细胞培养物中检测到，而Y2 mRNA仅限于脑。此外，NPY的表达在初代颅骨培养物中受到流体剪切应力时降低了30-40%。此外，用外源性NPY处理小鼠颅骨成骨细胞显示细胞内cAMP和成骨细胞分化标志物（骨钙素、BSP和DMP1）水平降低。这些结果强调了局部NPY信号对成骨细胞谱系分化的潜在调节。
Osteocytes are the most abundant osteoblast lineage cells within the bone matrix. They respond to mechanical stimulation and can participate in the release of regulatory proteins that can modulate the activity of other bone cells. We hypothesize that neuropeptide Y (NPY), a neurotransmitter with regulatory functions in bone formation, is produced by osteocytes and can affect osteoblast activity. To study the expression of NPY by the osteoblast lineage cells, we utilized transgenic mouse models in which we can identify and isolate populations of osteoblasts and osteocytes. The Col2.3GFP transgene is active in osteoblasts and osteocytes, while the DMP1 promoter drives green fluorescent protein (GFP) expression in osteocytes. Real-time PCR analysis of RNA from the isolated populations of cells derived from neonatal calvaria showed higher NPY mRNA in the preosteocytes/osteocytes fraction compared to osteoblasts. NPY immunostaining confirmed the strong expression of NPY in osteocytes (DMP1GFP+), and lower levels in osteoblasts. In addition, the presence of NPY receptor Y1 mRNA was detected in cavaria and long bone, as well as in primary calvarial osteoblast cultures, whereas Y2 mRNA was restricted to the brain. Furthermore, NPY expression was reduced by 30–40% in primary calvarial cultures when subjected to fluid shear stress. In addition, treatment of mouse calvarial osteoblasts with exogenous NPY showed a reduction in the levels of intracellular cAMP and markers of osteoblast differentiation (osteocalcin, BSP, and DMP1). These results highlight the potential regulation of osteoblast lineage differentiation by local NPY signaling.
登录
查看更多内容
影响因子：
7.7
作者：
la Fleur, SE;Ji, H;Dallman, MF
通讯作者：
Dallman, MF
影响因子：
4.8
作者：
SAR, M;SAHU, A;KALRA, SP
通讯作者：
KALRA, SP
影响因子：
6.2
作者：
Wadhwa, S;Godwin, SL;Pilbeam, CC
通讯作者：
Pilbeam, CC
影响因子：
6.2
作者：
Baldock, PA;Sainsbury, A;Gardiner, EM
通讯作者：
Gardiner, EM
影响因子：
3
作者：
ALLEN, BL;FERGUSON, RL;OBRIEN, RP
通讯作者：
OBRIEN, RP