A novel biotinylated degradable polymer for cell-interactive applications
A novel biotinylated degradable polymer for cell-interactive applications
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DOI：
10.1002/(sici)1097-0290(19980605)58:5
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发表时间：
1998-06-05
影响因子：
3.8
通讯作者：
Shakesheff, KM
中科院分区：
文献类型：
--
作者：
Cannizzaro, SM;Padera, RF;Shakesheff, KM
我们描述了一种新的生物可降解聚合物的发展，旨在目前在任何建筑材料的表面生物活性图案。该聚合物是生物素化聚乙二醇（PEG）与聚乳酸（PLA）的嵌段共聚物;它利用生物素-抗生物素蛋白系统的高亲和力偶联进行制造后表面工程。我们表明，使用表面等离子体共振分析（SPR）和共聚焦显微镜，表面工程可以在水溶液条件下在短时间内实现。这些表面与细胞表面分子相互作用并产生有益的反应，如整合素介导的内皮细胞在呈现RGD肽粘附序列的聚合物表面上的扩散的模型研究所证明的。(C)John Wiley & Sons，Inc.
We describe the development of a novel biodegradable polymer designed to present bioactive motifs at the surfaces of materials of any architecture. The polymer is a block copolymer of biotinylated poly(ethylene glycol) (PEG) with poly(lactic acid) (PLA); it utilizes the high-affinity coupling of the biotin-avidin system to undergo postfabrication surface engineering. We show, using surface plasmon resonance analysis (SPR) and confocal microscopy that surface engineering can be achieved under aqueous conditions in short time periods. These surfaces interact with cell surface molecules and generate beneficial responses as demonstrated by the model study of integrin-mediated spreading of endothelial cells on polymer surfaces presenting RGD peptide adhesion sequences. (C) 1998 John Wiley & Sons, Inc.