Detection and genotyping of astroviruses from children with acute gastroenteritis from Goiânia, Goiás, Brazil.
Detection and genotyping of astroviruses from children with acute gastroenteritis from Goiânia, Goiás, Brazil.
复制标题
巴西戈亚斯州戈亚尼亚急性胃肠炎儿童星状病毒的检测和基因分型。
DOI：
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发表时间：
2002
影响因子：
3.1
通讯作者：
J. P. Gagliardi Leite
中科院分区：
文献类型：
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作者：
Divina das Dores de Paula Cardoso;F. Fiaccadori;Menira Borges de Lima Dias e Souza;R. M. Bringel Martins;J. P. Gagliardi Leite
背景
星状病毒是儿童腹泻的重要病原体。我们研究的目的是检测巴西戈亚尼亚-戈亚斯急性胃肠炎患儿粪便样本中的星状病毒并对其进行基因分型。
材料/方法
样本采集自患有急性胃肠炎的5岁以下儿童，这些儿童在位于巴西中西部地区的戈亚尼亚-戈亚斯市的两家公立医院住院。从1998年3月至2000年3月，共收集了516个粪便样本。首先通过酶免疫测定法（EIARA）和PAGE检测轮状病毒筛选这些样本中的轮状病毒和腺病毒。在两种病毒阴性的所有样品（n=351）的星状病毒的存在进行了研究，通过逆转录-聚合酶链反应（RT-PCR）。
结果
从2岁以下儿童中获得的样本中有2.8%通过RT-PCR对星状病毒呈阳性，预期扩增子大小为449 bp。除一个样本外，所有阳性样本都是在1998年10月至12月期间采集的，这一时期正好是该地区的雨季（春季/初夏）。巢式PCR基因分型结果显示，所有样品均为星状病毒基因型1，扩增产物条带长度为212 bp。
结论
这项研究支持了关于急性胃肠炎儿童中星状病毒发生的文献数据。它还有助于更好地了解腹泻的病毒病因。
BACKGROUND
The astroviruses constitute important agents of childhood diarrhea. The purpose of our research was to detect and genotype astroviruses in fecal samples from children with acute gastroenteritis from Goiânia- Goiás, Brazil.
MATERIAL/METHODS
The samples were collected from children up to five years of age with acute gastroenteritis, hospitalized in two public hospitals in Goiânia- Goiás, a city located in the West Central region of Brazil. A total of 516 fecal samples were collected from March 1998 to March 2000. These samples were first screened for rotavirus and adenovirus by an enzyme immunoassay (EIARA) and by PAGE for the detection of rotaviruses. In all samples negative for both viruses (n=351) the presence of astrovirus was investigated by reverse-transcription-polymerase chain reaction (RT-PCR).
RESULTS
2.8% of the samples obtained from children up to two years of age were positive for astrovirus by RT-PCR, with an expected amplicon size of 449 bp. All positive samples, except one, were collected between October and December of 1998, which corresponds to the rainy season in the region (spring/early summer). Nested-PCR genotyping showed that all samples were genotyped and belonged to astrovirus genotype 1, presenting an amplicon band pattern of 212 bp.
CONCLUSIONS
This study supports data from the literature about the occurrence of astrovirus in children with acute gastroenteritis. It also helps provide a better understanding of viral etiology in diarrhea.