Flavonoids from Astragalus membranaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells
Flavonoids from Astragalus membranaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells
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DOI：
10.1007/s12272-013-0174-7
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发表时间：
2014-02-01
影响因子：
6.7
通讯作者：
Kim, Young Ho
中科院分区：
文献类型：
--
作者：
Li, Wei;Sun, Ya Nan;Kim, Young Ho
黄芪（黄芪）是一种重要的传统中药，被广泛用作补品，以增强人体的自然防御机制。在这项植物化学研究中，12种黄酮类化合物，异甘草素（1），甘草素（2），毛蕊异黄酮（3），毛蕊异黄酮7-O-β-d-葡萄糖苷（4），芒柄花素（5），芒柄花素7-O-β-d-葡萄糖苷（6），大豆苷元（7），大豆苷元7-O-β-d-葡萄糖苷（8），甲基尼梭素（9），甲基尼梭素3-O-β-d-葡萄糖苷（10），异苦豆醇（11），和isomucronulatol7-O-beta-d-glucoside（12）。膜壳菌通过光谱数据与文献报道值的比较，确定了它们的结构。研究了分离的化合物对脂多糖（LPS）刺激的骨髓来源的树突状细胞的影响。化合物1和2对LPS诱导的IL-6和IL-12 p40产生表现出显著的抑制作用，IC 50值范围为2.7至6.1 μ M。化合物1还显示出对LPS刺激的TNF-α产生的中等抑制作用，IC 50值为20.1 μ M。进一步的研究潜在的抗炎作用和从A类黄酮的好处。膜是必要的。
Radix Astragali (Astragalus membranaceus) is an important traditional Chinese medicine that is widely used as a tonic to enhance the body's natural defense mechanisms. In this phytochemical study, 12 flavonoids, isoliquiritigenin (1), liquiritigenin (2), calycosin (3), calycosin 7-O-beta-d-glucoside (4), formononetin (5), formononetin 7-O-beta-d-glucoside (6), daidzein (7), daidzein 7-O-beta-d-glucoside (8), methylnissolin (9), methylnissolin 3-O-beta-d-glucoside (10), isomucronulatol (11), and isomucronulatol 7-O-beta-d-glucoside (12), were isolated from the roots of A. membranaceus. Their structures were elucidated by comparing spectroscopic data with reported values. The effects of the isolated compounds on lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells were investigated. Compounds 1 and 2 exhibited significant inhibitory effects on LPS-induced IL-6 and IL-12 p40 production, with IC50 values ranging from 2.7 to 6.1 mu M. Compound 1 also showed a moderate inhibitory effect on LPS-stimulated production of TNF-alpha with an IC50 value of 20.1 mu M. Further studies of the potential anti-inflammatory effects and benefits of flavonoids from A. membranaceus are warranted.