PROLINE ACCUMULATION AND ITS IMPLICATION IN COLD TOLERANCE OF REGENERABLE MAIZE CALLUS
PROLINE ACCUMULATION AND ITS IMPLICATION IN COLD TOLERANCE OF REGENERABLE MAIZE CALLUS
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DOI：
10.1104/pp.83.3.703
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发表时间：
1987-03-01
期刊：
影响因子：
7.4
通讯作者：
WIDHOLM, JM
中科院分区：
文献类型：
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作者：
DUNCAN, DR;WIDHOLM, JM
玉米（Zea mays L.）当经受甘露醇诱导的水分胁迫、冷却温度（19 ℃）、温度（10 ℃）、温度（10 ℃）和温度（10 ℃）时，近交系B37wx、H99、H993H95、Mo17和Pa91积累的脯氨酸达到对照愈伤组织的2.1至2.5倍水平。C）和脱落酸（阿坝）。0.53摩尔甘露醇加0.1毫摩尔阿坝的组合诱导的脯氨酸积累约为对照愈伤组织的4.5倍，相当于每克愈伤组织鲜重约0.18毫摩尔脯氨酸。脯氨酸的积累与培养基中甘露醇的水平直接相关。阿坝的水平大于1.0微摩尔所需的介质中诱导脯氨酸积累甘露醇诱导的。诱导脯氨酸最大积累的甘露醇和ABA水平也抑制愈伤组织的生长并提高其耐冷性。在培养基中加入脯氨酸（12毫摩尔）也可使愈伤组织的耐受性提高到4.0 ℃。C.由甘露醇和阿坝组合诱导的耐冷性增加允许玉米自交系A632、A634 Ht、B37 wx、C103 DTrf、Fr27 rhm、H99、Pa 91、Va35和W117 Ht在4度下储存。C培养90天，是典型愈伤组织存活时间的两倍多。这些研究表明，脯氨酸和诱导脯氨酸积累的条件增加了可再生玉米愈伤组织的耐冷性。
Embryogenic callus of maize (Zea mays L.) inbreds B37wx, H99, H993H95, Mo17, and Pa91 accumulated proline to levels 2.1 to 2.5 times that of control callus when subjected to mannitol-induced water stress, cool temperatures (19.degree. C) and abscisic acid (ABA). A combination of 0.53 molar mannitol plus 0.1 millimolar ABA induced a proline accumulation to about 4.5 times that of control callus, equivalent to approximately 0.18 millimoles proline per gram fresh weight of callus. Proline accumulation was directly related to the level of mannitol in the medium. Levels of ABA greater than 1.0 micromolar were required in the medium to induce proline accumulation comparable to that induced by mannitol. Mannitol and ABAlevels that induced maximum accumulation of proline also inhibited callus growth and increased tolerance to cold. Proline (12 millimolar) added to the culture media also increased the tolerance of callus to 4.degree. C. The increased cold tolerance induced by the combination of mannitol and ABA has permitted the storage of the maize inbreds A632, A634Ht, B37wx, C103DTrf, Fr27rhm, H99, Pa91, Va35, and W117Ht at 4.degree. C for 90 days which is more than double the typical survival time of callus. These studies show that proline and conditions which induce proline accumulation increase the cold tolerance of regenerable maize callus.