Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice.
Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice.
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CNS星形胶质细胞在AldH1L1-Creert2 BAC转基因小鼠中的诱导靶向。
DOI：
10.12688/f1000research.10509.1
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Saher G
中科院分区：
文献类型：
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作者：
Winchenbach J;Düking T;Berghoff SA;Stumpf SK;Hülsmann S;Nave KA;Saher G
背景：由于缺乏在整个中枢神经系统（包括新皮层）有效表达可诱导的Cre重组酶的遗传工具，对具有高级脑功能的星形胶质细胞的研究一直受到阻碍。方法：因此，我们产生了在Aldh1l1调控区控制下表达CreERT2的BAC转基因小鼠。结果：当与Cre报告小鼠杂交时，成年Aldh1l1-CreERT2小鼠在星形胶质细胞中表现出有效的基因靶向性。在中枢神经系统神经元、少突胶质细胞和小胶质细胞中未检测到这种cre介导的重组。正如预期的那样，Aldh1l1-CreERT2在包括肝脏和肾脏在内的几个外周器官中表达明显。结论：综上所述，Aldh1l1-CreERT2小鼠是研究星形胶质细胞在神经血管耦合、脑代谢、突触可塑性等神经-胶质相互作用方面的有用工具。
Background: Studying astrocytes in higher brain functions has been hampered by the lack of genetic tools for the efficient expression of inducible Cre recombinase throughout the CNS, including the neocortex. Methods: Therefore, we generated BAC transgenic mice, in which CreERT2 is expressed under control of the Aldh1l1 regulatory region. Results: When crossbred to Cre reporter mice, adult Aldh1l1-CreERT2 mice show efficient gene targeting in astrocytes. No such Cre-mediated recombination was detectable in CNS neurons, oligodendrocytes, and microglia. As expected, Aldh1l1-CreERT2 expression was evident in several peripheral organs, including liver and kidney. Conclusions: Taken together, Aldh1l1-CreERT2 mice are a useful tool for studying astrocytes in neurovascular coupling, brain metabolism, synaptic plasticity and other aspects of neuron-glia interactions.