Morphologically and Functionally Distinct Lipid Droplet Subpopulations.
Morphologically and Functionally Distinct Lipid Droplet Subpopulations.
复制标题
形态和功能不同的脂滴亚群
DOI：
10.1038/srep29539
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发表时间：
2016-07-08
影响因子：
4.6
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Zhang S;Wang Y;Cui L;Deng Y;Xu S;Yu J;Cichello S;Serrero G;Ying Y;Liu P
脂滴（Lipid droplet，LD）是一种多功能的细胞器，在同一细胞中，它与其他细胞膜结构结合，大小不一，这可能与其功能多样性有关。在这里，我们建立了一种方法，分离的CHO K2 LD LD LD亚群分为三个不同的大小类别。具有最小LD的亚群几乎不含ER和其他膜结构，而具有较大LD的亚群含有完整的ER。这些不同的LD亚群的蛋白质组成和招募蛋白质的能力不同。该方法也适用于其他来源的LD，如Huh 7细胞，小鼠肝脏和棕色脂肪组织等。我们开发了一种只需要分离的LD，辅酶A和ATP来驱动脂质合成的体外测定法。LD亚群几乎耗尽ER能够将脂肪酸纳入三酰甘油和磷脂。总之，我们的数据表明，LD在一个给定的细胞是异质性的大小和功能，并建议LD是细胞的脂质合成细胞器之一。
Lipid droplet (LD), a multi-functional organelle, is often found to associate with other cellular membranous structures and vary in size in a given cell, which may be related to their functional diversity. Here we established a method to separate LD subpopulations from isolated CHO K2 LDs into three different size categories. The subpopulation with smallest LDs was nearly free of ER and other membranous structures while those with larger LDs contained intact ER. These distinct subpopulations of LDs differed in their protein composition and ability to recruit proteins. This method was also applicable to LDs obtained from other sources, such as Huh7 cells, mouse liver and brown adipose tissue,et al. We developed anin vitroassay requiring only isolated LDs, Coenzyme A and ATP to drive lipid synthesis. The LD subpopulation nearly depleted of ER was able to incorporate fatty acids into triacylglycerol and phospholipids. Together, our data demonstrate that LDs in a given cell are heterogeneous in size and function and suggest that LDs are one of cellular lipid synthetic organelles.