Analysis of effectiveness of R1-nj anthocyanin marker for in vivo haploid identification in maize and molecular markers for predicting the inhibition of R1-nj expression
Analysis of effectiveness of R1-nj anthocyanin marker for in vivo haploid identification in maize and molecular markers for predicting the inhibition of R1-nj expression
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DOI：
10.1007/s00122-014-2419-3
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发表时间：
2015-01-01
影响因子：
5.4
通讯作者：
Boddupalli, Prasanna M.
中科院分区：
文献类型：
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作者：
Chaikam, Vijay;Nair, Sudha K.;Boddupalli, Prasanna M.
在热带玉米种质中，R1-nj花色素苷标记的抑制现象非常普遍，严重影响了单倍体鉴定的效率。本研究筛选出了能可靠区分花色苷颜色抑制剂种质的分子标记，R1-Navajo（R1-nj）颜色标记有助于在玉米体内单倍体诱导过程中，在种子期就能方便、快速地鉴定出单倍体籽粒。然而，纳瓦霍表型在一些种质中可以被完全抑制或表达不良，使得在种子阶段鉴定单倍体是不可能的或效率低下的。在这项研究中，我们的特点是R1-nj标记的表达在一个大阵列的热带/亚热带自交系，育种群体和地方品种通过与R1-nj为基础的热带化单倍体诱导杂交。在热带育种计划中使用的玉米自交系中，纳瓦霍表型的抑制频率很高。全基因组关联作图表明，C1花青素调控位点是影响抑制纳瓦霍表型的最重要的遗传因素。根据C1与C1-I等位基因的多态性设计分子标记检测。一组714个自交系的分析表明，两个基因特异性标记的组合-8 bp的C1-I InDel和C1-I SNP-可以预测与高精度的存在下，花色素苷颜色抑制的种质分析。在这项研究中产生的信息有助于作出明智的决定，在热带种质，特别是那些来自CIMMYT的精英玉米系的双单倍体（DH）系开发的源种群的构成。鉴定和验证的C1-I基因特异性分子标记将有助于高通量和成本效益的评价大库种质的存在下，在玉米种质的显性颜色抑制剂。
R1-nj anthocyanin marker inhibition is highly frequent in tropical maize germplasm considerably affecting efficiency of haploid identification. Molecular markers reliably differentiating germplasm with anthocyanin color inhibitor have been identified in this study.The R1-Navajo (R1-nj) color marker facilitates easy and quick identification of haploid kernels at the seed stage during in vivo haploid induction process in maize. However, the Navajo phenotype can be completely suppressed or poorly expressed in some germplasm, making it impossible or inefficient to identify haploids at the seed stage. In this study, we characterized the expression of R1-nj marker in a large array of tropical/subtropical inbred lines, breeding populations and landraces by crossing with the R1-nj-based tropicalized haploid inducer. There was a high frequency of inhibition of the Navajo phenotype in the maize inbred lines, which are used in tropical breeding programs. Genome-wide association mapping showed that the C1 anthocyanin regulatory locus is the most significant genetic factor influencing inhibition of the Navajo phenotype. Molecular marker assays were designed based on polymorphism in the C1 vs C1-I alleles. Analysis of a set of 714 inbred lines demonstrated that a combination of two gene-specific markers-8 bp C1-I InDel and C1-I SNP-could predict with high accuracy the presence of anthocyanin color inhibition in the germplasm analyzed. Information generated in this study aids in making informed decisions on the constitution of source populations for doubled haploid (DH) line development in tropical germplasm, particularly those derived from elite maize lines from CIMMYT. The C1-I gene-specific molecular markers identified and validated will facilitate high-throughput and cost-effective evaluation of a large pool of germplasm for the presence of the dominant color inhibitor in maize germplasm.