Expression of angiogenesis associated matrix metalloproteinases and extracellular matrix proteins in cerebral vascular malformations
Expression of angiogenesis associated matrix metalloproteinases and extracellular matrix proteins in cerebral vascular malformations
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DOI：
10.1016/j.jocn.2009.06.008
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发表时间：
2010-02-01
影响因子：
2
通讯作者：
Kilic, Turker
中科院分区：
文献类型：
--
作者：
Bicer, Atilla;Guclu, Bulent;Kilic, Turker
本研究旨在比较脑动静脉畸形（AVM）和脑海绵状血管畸形（CCM）的基质金属蛋白酶（MMP）和选定的细胞外基质（ECM）蛋白的免疫组化表达，这些蛋白在血管生成的调节中发挥作用。AVM（n = 14）和CCM（n = 15）患者的新鲜冷冻手术标本用MMP-2、MMP-9、层粘连蛋白、纤连蛋白和腱生蛋白的抗体进行化学染色。为了比较AVM和CCM，使用病变的每个组织学层的四点评分系统对每种蛋白质的表达进行分级。MMP-2和MMP-9在CCM的血管壁中的表达比AVM的所有可比层（内皮、内皮下和血管周围空间）更强。与AVM相比，CCM中与早期血管生成相关的MMP和其他ECM蛋白的表达更强，这可能支持CCM发生在比AVM更早的胚胎发生阶段的假设。(C)2009爱思唯尔有限公司保留所有权利。
This study aimed to compare cerebral arteriovenous malformations (AVM) and cerebral cavernous malformations (CCM) with regard to the immunohistochemical expressions of matrix metalloproteinases (MMP) and selected extracellular matrix (ECM) proteins, which have a role in the regulation of angiogenesis. Fresh-frozen surgical specimens from patients with AVM (n = 14) and CCM (n = 15) were immunohistochemically stained with antibodies for MMP-2, MMP-9, laminin, fibronectin and tenascin. To compare AVM and CCM, expression of each protein was graded using a four-point scoring system for each histological layer of the lesion. MMP-2 and MMP-9 were more strongly expressed in the vascular walls of CCMs compared to AVMs for all comparable layers: endothelium, subendothelium and the perivascular space. The stronger expression of MMP and other ECM proteins associated with early angiogenesis in CCMs compared to AVMs may support the hypothesis that CCMs occur at an earlier embryogenic stage than AVMs. (C) 2009 Elsevier Ltd. All rights reserved.