Vaginal epithelial dendritic cells renew from bone marrow precursors
Vaginal epithelial dendritic cells renew from bone marrow precursors
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DOI：
10.1073/pnas.0707179104
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发表时间：
2007-11-27
影响因子：
11.1
通讯作者：
Iwasaki, Akiko
中科院分区：
文献类型：
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作者：
Iijima, Norifumi;Linehan, Melissa M.;Iwasaki, Akiko
树突状细胞(DC)是一种重要的专职抗原提呈细胞，能够启动初始免疫反应。树突状细胞的一个特殊亚群，朗格汉斯细胞(LCS)，位于皮肤的层状鳞状上皮层以及阴道和口腔的粘膜上皮层内。阴道粘膜在性激素的控制下经历周期性变化，阴道上皮树突状细胞(VEDCs)的更新特性尚不清楚。在这里，我们研究了VEDCs的起源。与皮肤表皮LC相比，阴道上皮中的DC主要由非单核细胞来源的骨髓来源的前体细胞再生，在稳态条件下半衰期为13天。感染HSV-2后，Gr-1(Hi)单核细胞可产生VEDCs。此外，流式细胞术分析显示至少存在三个不同的群体，即CD11b(+)F4/80(Hi)、ClD11b(+)F4/80(Int)和CD11b(-)F4/80(-)。重要的是，这些VEDC群体低水平表达CD207，并且与皮肤LC相比，具有结构性更高的活化表型。总的来说，我们的结果揭示了VEDCs在其表型、激活状态和内环境平衡更新潜力方面的粘膜特异性特征。
Dendritic cells (DCs) represent key professional antigen-presenting cells capable of initiating primary immune responses. A specialized subset of DCs, the Langerhans cells (LCs), are located in the stratified squamous epithelial layer of the skin and within the mucosal epithelial lining of the vaginal and oral cavities. The vaginal mucosa undergoes cyclic changes under the control of sex hormones, and the renewal characteristics of the vaginal epithelial DCs (VEDCs) remain unknown. Here, we examined the origin of VEDCs. In contrast to the skin epidermal LCs, the DCs in the epithelium of the vagina were found to be repopulated mainly by nonmonocyte bone-marrow-derived precursors, with a half-life of 13 days under steady-state conditions. Upon infection with HSV-2, the Gr-1(hi) monocytes were found to give rise to VEDCs. Furthermore, flow cytometric analysis of the VEDCs revealed the presence of at least three distinct populations, namely, CD11b(+)F4/80(hi), ClD11b(+)F4/80(int), and CD11b(-)F4/80(-). Importantly, these VEDC populations expressed CD207 at low levels and had a constitutively more activated phenotype compared with the skin LCs. Collectively, our results revealed mucosa-specific features of the VEDCs with respect to their phenotype, activation status,and homeostatic renewal potential.