Boron demanding tissues of Brassica napus express specific sets of functional Nodulin26-like Intrinsic Proteins and BOR1 transporters
Boron demanding tissues of Brassica napus express specific sets of functional Nodulin26-like Intrinsic Proteins and BOR1 transporters
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DOI：
10.1111/tpj.14428
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发表时间：
2019-07-15
期刊：
影响因子：
7.2
通讯作者：
Bienert, Gerd Patrick
中科院分区：
文献类型：
--
作者：
Diehn, Till Arvid;Bienert, Manuela Desiree;Bienert, Gerd Patrick
植物对必需元素硼（B）的吸收和转运受到两个跨膜转运蛋白家族的调控：Nodulin 26-like Intrinsic Protein（NIP）和BOR转运蛋白家族。尽管农业上重要的作物油菜对B缺乏高度敏感，并且已表明NIP和BOR对该物种的B效率负责，但这些转运蛋白亚家族的功能信息极其罕见。在这里，我们的分子特征的NIP和BOR 1转运蛋白家族在欧洲冬季型CV。Darmor-PBY018.我们在异源卵母细胞和酵母表达系统中的转运试验以及在植物生长互补试验中证明了NIP 5、NIP 6、NIP 7和BOR 1同种型的B转运活性。此外，我们提供的功能和定量证据表明，NIP 2，NIP 3和NIP 4组的成员也促进了B的运输。通过定量聚合酶链反应生成详细的B和组织依赖性B转运蛋白表达图。我们发现，NIP 5亚型在B缺乏的条件下，在根，但也在地上部组织中高度上调。此外，我们检测到转录的几个B-渗透NIP从不同的群体在花组织中，有助于B分布在高度B缺乏敏感的花。
The sophisticated uptake and translocation regulation of the essential element boron (B) in plants is ensured by two transmembrane transporter families: the Nodulin26-like Intrinsic Protein (NIP) and BOR transporter family. Though the agriculturally important crop Brassica napus is highly sensitive to B deficiency, and NIPs and BORs have been suggested to be responsible for B efficiency in this species, functional information of these transporter subfamilies is extremely rare. Here, we molecularly characterized the NIP and BOR1 transporter family in the European winter-type cv. Darmor-PBY018. Our transport assays in the heterologous oocyte and yeast expression systems as well as in growth complementation assays in planta demonstrated B transport activity of NIP5, NIP6, NIP7 and BOR1 isoforms. Moreover, we provided functional and quantitative evidence that also members of the NIP2, NIP3 and NIP4 groups facilitate the transport of B. A detailed B- and tissue-dependent B-transporter expression map was generated by quantitative polymerase chain reaction. We showed that NIP5 isoforms are highly upregulated under B-deficient conditions in roots, but also in shoot tissues. Moreover, we detected transcripts of several B-permeable NIPs from various groups in floral tissues that contribute to the B distribution within the highly B deficiency-sensitive flowers.