Chlorpromazine metabolism IV: quaternization as a key to determination of picomoles of chlorpromazine and other tertiary amine drugs.
Chlorpromazine metabolism IV: quaternization as a key to determination of picomoles of chlorpromazine and other tertiary amine drugs.
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氯丙嗪代谢IV：季铵化是氯丙嗪和其他叔胺类药物皮摩尔含量测定的关键。
DOI：
10.1002/jps.2600640611
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
P. N. Kaul
中科院分区：
文献类型：
--
作者：
R. Lehr;P. N. Kaul
氯丙嗪与9-溴甲基吖啶在适当的条件下反应，生成一种无荧光的季铵衍生物，在随后的光解中释放荧光。这种荧光的主要成分是9-甲基吖啶（86%），而两个次要成分是9-吖啶甲醛（6%）和9-吖啶甲醇（8%）。导致这些产物形成的光解机制似乎涉及季盐的均裂和异裂。季铵化和光解都是化学计量的。荧光的适当分离及其定量测定构成了适用于氯丙嗪和其他叔胺药物的新的和高灵敏度的测定的基础。
Reaction of chlorpromazine with 9-bromomethylacridine under appropriate conditions yields a nonfluorescent quaternary ammonium derivative which, on subsequent photolysis, liberates fluorescence. The major component of this fluorescence is 9-methylacridine (86%), while two minor components are 9-acridinecarboxaldehyde (6%) and 9-acridinemethanol (8%). The mechanism of photolysis leading to formation of these products appears to involve homolytic as well as heterolytic cleavages of the quaternary salt. Both the quaternization and the photolysis are stoichiometric. Appropriate isolation of the fluorescence and its quantitative determination constitutes the basis of a new and highly sensitive assay applicable to chlorpromazine and other tertiary amine drugs.