Advanced mass spectrometry-based methods for the analysis of conformational integrity of biopharmaceutical products.
Advanced mass spectrometry-based methods for the analysis of conformational integrity of biopharmaceutical products.
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DOI：
10.2174/138920111798357311
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发表时间：
2011-10
影响因子：
2.8
通讯作者：
Kaltashov IA
中科院分区：
文献类型：
--
作者：
Bobst CE;Kaltashov IA
质谱已经成为生物制药行业分析仪器中不可或缺的工具，尽管其目前的用途仅限于表征重组蛋白药物的共价结构。然而，基于质谱的方法的应用范围开始扩展到包括生物制药产品的高阶结构和动力学的表征，这是由基于质谱的方法研究高阶蛋白质结构的最新进展所催化的发展。这两个特别有前途的方法，可能有最显着和持久的影响，在许多领域的生物制药分析，直接ESI MS和氢/氘交换，是本文的重点。
Mass spectrometry has already become an indispensable tool in the analytical armamentarium of the biopharmaceutical industry, although its current uses are limited to characterization of covalent structure of recombinant protein drugs. However, the scope of applications of mass spectrometry-based methods is beginning to expand to include characterization of the higher order structure and dynamics of biopharmaceutical products, a development which is catalyzed by the recent progress in mass spectrometry-based methods to study higher order protein structure. The two particularly promising methods that are likely to have the most significant and lasting impact in many areas of biopharmaceutical analysis, direct ESI MS and hydrogen/deuterium exchange, are focus of this article.