Reassortment between Avian H5N1 and Human H3N2 Influenza Viruses in Ferrets: a Public Health Risk Assessment
Reassortment between Avian H5N1 and Human H3N2 Influenza Viruses in Ferrets: a Public Health Risk Assessment
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DOI：
10.1128/jvi.00534-09
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发表时间：
2009-08-15
影响因子：
5.4
通讯作者：
Donis, Ruben O.
中科院分区：
文献类型：
--
作者：
Jackson, Sara;Van Hoeven, Neal;Donis, Ruben O.
本研究探讨了H5亚型人禽流感病毒能否在体内产生传播。为此，雪貂被最近禽H5 N1（A/Thailand/16/04）和人H3 N2（A/怀俄明州/3/03）病毒共感染。从合并感染的雪貂鼻分泌物的空斑纯化病毒的基因型分析显示，约9%的回收的病毒含有来自两种祖病毒的基因。H5和H3亚型病毒，包括reflectants，被发现在气道延伸到上呼吸道的雪貂。然而，在肺组织中仅发现亲本H5 N1基因型病毒。大约34%的回收的重组病毒具有H5血凝素（HA）基因，回收了5种独特的H5亚型。选择这些H5抗性菌株进行进一步研究，以检查它们的生长和传递特性。与亲本H5 N1病毒相比，五种具有代表性抗性基因型的H5病毒在感染雪貂的鼻分泌物中显示出降低的滴度。没有观察到感染和未感染的雪貂之间通过直接接触传播。这些研究表明，H5 N1禽流感病毒和H3 N2人类病毒之间的重组很容易发生在体内，而且这两种病毒亚型之间的重组很可能发生在雪貂上呼吸道。鉴于雪貂上呼吸道组织中的耐药病毒发生率相对较高，可以合理地得出结论，人类和动物与季节性流感病毒一起持续暴露于H5 N1会增加产生H5亚型耐药病毒的风险，这些病毒可能从上呼吸道分泌物中脱落。
This study investigated whether transmissible H5 subtype human-avian reassortant viruses could be generated in vivo. To this end, ferrets were coinfected with recent avian H5N1 (A/Thailand/16/04) and human H3N2 (A/Wyoming/3/03) viruses. Genotype analyses of plaque-purified viruses from nasal secretions of coinfected ferrets revealed that approximately 9% of recovered viruses contained genes from both progenitor viruses. H5 and H3 subtype viruses, including reassortants, were found in airways extending toward and in the upper respiratory tract of ferrets. However, only parental H5N1 genotype viruses were found in lung tissue. Approximately 34% of the recovered reassortant viruses possessed the H5 hemagglutinin (HA) gene, with five unique H5 subtypes recovered. These H5 reassortants were selected for further studies to examine their growth and transmissibility characteristics. Five H5 viruses with representative reassortant genotypes showed reduced titers in nasal secretions of infected ferrets compared to the parental H5N1 virus. No transmission by direct contact between infected and naive ferrets was observed. These studies indicate that reassortment between H5N1 avian influenza and H3N2 human viruses occurred readily in vivo and furthermore that reassortment between these two viral subtypes is likely to occur in ferret upper airways. Given the relatively high incidence of reassortant viruses from tissues of the ferret upper airway, it is reasonable to conclude that continued exposure of humans and animals to H5N1 alongside seasonal influenza viruses increases the risk of generating H5 subtype reassortant viruses that may be shed from upper airway secretions.