Strategies for Analyzing Neuronal Progenitor Development and Neuronal Migration in the Developing Cerebral Cortex
Strategies for Analyzing Neuronal Progenitor Development and Neuronal Migration in the Developing Cerebral Cortex
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DOI：
10.1093/cercor/bhq197
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发表时间：
2011-07-01
期刊：
影响因子：
3.7
通讯作者：
Anton, E. S.
中科院分区：
文献类型：
--
作者：
Higginbotham, Holden;Yokota, Yukako;Anton, E. S.
发育中的大脑皮层中功能性神经元连接的出现取决于1）神经祖细胞分化，其导致产生适当数量和类型的神经元，以及2）神经元迁移，其使得神经元能够适当定位，使得神经元之间的功能性突触连接的正确模式可以出现。在这篇综述中，我们讨论了1）目前可用的方法来研究神经祖细胞的发育和分化在发育中的大脑皮层和新兴技术在这方面，2）测定研究祖细胞的后代（即，神经元）的体外和体内的研究，以及3）使用这些测定来探测发育中的脑中这些事件的分子控制和评估人类神经发育障碍中被破坏的基因功能。
The emergence of functional neuronal connectivity in the developing cerebral cortex depends on 1) neural progenitor differentiation, which leads to the generation of appropriate number and types of neurons, and 2) neuronal migration, which enables the appropriate positioning of neurons so that the correct patterns of functional synaptic connectivity between neurons can emerge. In this review, we discuss 1) currently available methods to study neural progenitor development and differentiation in the developing cerebral cortex and emerging technologies in this regard, 2) assays to study the migration of descendents of progenitors (i.e., neurons) in vitro and in vivo, and 3) the use of these assays to probe the molecular control of these events in the developing brain and evaluation of gene functions disrupted in human neurodevelopmental disorders.