Successful cryopreservation of suspension cells by encapsulation-dehydration
Successful cryopreservation of suspension cells by encapsulation-dehydration
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通过封装脱水成功冷冻保存悬浮细胞
DOI：
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发表时间：
1995
影响因子：
3
通讯作者：
J. Dereuddre
中科院分区：
文献类型：
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作者：
Y. Bachiri;C. Gazeau;J. Hansz;C. Morisset;J. Dereuddre
将花楸花细胞悬液包封在海藻酸珠中进行低温保存。包被的细胞在蔗糖富集培养基中预培养数天，在硅胶上干燥，并直接在液氮中冷却。在室温下在空气中再加热后，将海藻酸珠放在半固体培养基上。再生后，将微珠转移到液体培养基中产生新的细胞悬浮液。细胞的存活和再生取决于预培养时间和风干后的剩余水分含量。
Cryopreservation of a Catharanthus cell suspension was performed after encapsulation in alginate beads. Encapsulated cells were precultured in sucrose-enriched medium for several days, dried over silica gel, and directly cooled in liquid nitrogen. After rewarming in air at room temperature, alginate beads were placed on semi-solid culture medium. Following regrowth, beads transferred to liquid medium generated a new cell suspension. Cell survival and regrowth from cryopreserved encapsulated cells depended on preculture duration and residual water content after air-drying.