Circulatory miR-628-5p is downregulated in prostate cancer patients.
Circulatory miR-628-5p is downregulated in prostate cancer patients.
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DOI：
10.1007/s13277-014-1638-1
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发表时间：
2014-05
期刊：
影响因子：
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通讯作者：
Kumar D
中科院分区：
文献类型：
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作者：
Srivastava A;Goldberger H;Dimtchev A;Marian C;Soldin O;Li X;Collins SP;Suy S;Kumar D
前列腺癌（PCa）是美国男性的主要健康问题。microRNA（miRNAs）的异常表达与多种癌症（包括PCa）的发病机制相关。已在血清中检测到循环形式的miRNA，并有望作为微创癌症生物标志物。本研究旨在鉴定潜在的循环miRNAs，这些miRNAs可以为PCa的临床诊断提供新的机制，并可以作为潜在的生物标志物和/或治疗靶点。候选血清miRNA检测通过使用PCR微阵列在学习集的六个非洲裔美国人（AA）和六个白人美国人（CA）PCa患者。通过qRT-PCR在来自36个AA（24个PCa患者和12个对照）和36个CA（16个PCa患者和20个对照）的血清样品中验证候选miRNA的区分性能。从miRNA谱分析实验中，选择三种差异表达的miRNA（miR-25、miR-101和miR-628- 5 p）用于未来验证。在验证集中，与正常个体相比，PCa患者血清中miR-25（p<0.01）、miR-101（p<0.001）和miR-628- 5 p（p<0.0001）的总体低表达。基于种族的细分显示，与各自的对照组相比，AA和CA PCa患者的血清miR-628- 5 p表达水平显著下调。我们的研究结果表明，这三种miRNA，特别是miR-628- 5 p，可以进一步开发为生物标志物，可以作为新的非侵入性生物标志物用于PCa的诊断和预后。
Prostate cancer (PCa) is a major health concern for men in the USA. Aberrant expression of microRNAs (miRNAs) has been associated with the pathogenesis of various cancers, including PCa. Circulatory forms of miRNAs have been detected in serum and hold promise as minimally invasive cancer biomarkers. This study aimed to identify potential circulatory miRNAs that can provide insights into new mechanisms for clinical diagnosis of PCa and can serve as potential biomarkers and/or therapeutic targets. Candidate serum miRNAs were detected by using PCR microarray in a learning set of six African American (AA) and six Caucasian American (CA) PCa patients. Discriminating performance of candidate miRNAs was validated by qRT-PCR in serum samples from 36 AA (24 PCa patients and 12 controls) and 36 CA (16 PCa patients and 20 controls). From the miRNA profiling experiments, three differentially expressed miRNAs (miR-25, miR-101, and miR-628-5p) were selected for future validation. In the validation set, there was an overall low expression of miR-25 (p<0.01), miR-101 (p<0.001), and miR-628-5p (p<0.0001) in serum of PCa patients as compared with normal individuals. Subdivision on the basis of ethnicity showed that serum expression levels of miR-628-5p were significantly downregulated in both AA and CA PCa patients when compared with their respective controls. Our results demonstrate that the three miRNAs, particularly miR-628-5p, may be further developed as a biomarker, which can serve as novel noninvasive biomarker for PCa diagnosis and prognosis.