A simple method for high-throughput extract prefractionation for biological screening
A simple method for high-throughput extract prefractionation for biological screening
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DOI：
10.2533/chimia.2007.327
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发表时间：
2007-01-01
期刊：
影响因子：
1.2
通讯作者：
Butler, Mark S.
中科院分区：
文献类型：
--
作者：
Appleton, David R.;Buss, Antony D.;Butler, Mark S.
The screening of crude natural product extracts can be problematic, especially in enzyme/protein-based assays, due to promiscuous inhibitors and interference compounds, while high salt and lipid content can significantly dilute compounds of interest. Described herein is a simple method for high-throughput fractionation of crude extracts that can help address some of these issues. Results obtained indicate a significant improvement in assay hit rates without a reduction of hit quality and show how prefractionation aids the discovery of compounds that would be undetected by screening crude extracts alone.
