Somatostatin inhibits pro-inflammatory cytokine secretion from rat hepatic stellate cells
Somatostatin inhibits pro-inflammatory cytokine secretion from rat hepatic stellate cells
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DOI：
10.1111/j.1478-3231.2005.01057.x
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发表时间：
2005-08-01
影响因子：
6.7
通讯作者：
Chowers, Y
中科院分区：
文献类型：
--
作者：
Lang, A;Sakhnini, E;Chowers, Y
背景/目的：活化的肝星状细胞(HSCs)与肝纤维化有关。生长抑素(SOM)具有免疫调节作用。本研究的目的是评估HSCs分泌促炎细胞因子的能力，并确定SOM对这些介质分泌的影响。方法：用双抗体夹心法检测活化的大鼠肝星状细胞分泌IL-1β、IL-8和肿瘤坏死因子-α的能力。用RNA保护法检测细胞因子的mRNA水平。与SOM或奥曲肽孵育后，检测趋化因子和细胞因子mRNA的表达及这些介质的分泌。结果：HSCs可自发分泌IL-1β、IL-8和TNF-α。IL-1β和肿瘤坏死因子-α刺激后，这种分泌增加。SOM可抑制IL-1β、IL-8和TNF-α的自发分泌，并抑制IL-1β和TNF-α的表达。奥曲肽抑制IL-1β和IL-8的分泌。结论：SOM对HSCs具有抑制免疫调节作用。
Background/aims: Activated hepatic stellate cells (HSCs) have been implicated in hepatic fibrosis. Somatostatin (SOM) has an immunomodulatory role. The aim of this study was to assess the secretion of pro-inflammatory cytokines by HSCs and to determine the effect of SOM on the secretion of these mediators. Methods: Activated rat HSCs were evaluated for their secretion of IL-1 beta, IL-8, and TNF-alpha using ELISA. RNA protection assay was used to determine cytokine mRNA levels. The expression of chemokine and cytokine mRNA and the secretion of these mediators were assessed following incubation with SOM or octreotide. Results: HSCs spontaneously secreted IL-1 beta, IL-8, and TNF-alpha. This secretion was augmented following stimulation by IL-1 beta and TNF-alpha. SOM inhibited the spontaneous and TNF-alpha-induced secretion of IL-1 beta, IL-8, and TNF-alpha and suppressed the expression of IL-1 beta and TNF-alpha mRNA. Octreotide suppressed the secretion of IL-1 beta and IL-8. Conclusions: These observations indicate that SOM exerts an inhibitory immunomodulatory effect on HSCs.